17-1010 | Magna ChIP-Seq™ ChIP and NGS Library Preparation Kit

17-1010
10 library constructions  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      ChIP-seq, Sample Prep
      Description
      Catalogue Number17-1010
      Trade Name
      • Magna ChIP
      • Upstate
      DescriptionMagna ChIP-Seq™ ChIP and NGS Library Preparation Kit
      OverviewChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP-Seq has become an indispensable tool. The Magna ChIP-Seq Kit simplifies this technique by providing a validated set of reagents, easy-to-follow protocols, controls, and in process quality control guidelines to allow virtually any laboratory to conduct genome-wide analysis of DNA-protein interactions and the profiling of epigenetic marks.

      • Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin
      • Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA
      • Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days
      • Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class
      • Enzymes and buffers provided in convenient master mix formulations streamlines library construction
      • Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set
      • Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer II

      Materials Required but Not DeliveredCells, stimulated or treated as desired
      Antibody for chromatin immunoprecipitation
      37% Formaldehyde
      Taq DNA polymerase and dNTPs
      Master Mix for qPCR
      DNase and RNase-free sterile H2O
      Quant-iT™ dsDNA HS Assay Kit, (Life Technologies catalog #: Q32851)
      Oligonucleotide primers and adaptors
      Low Elution Volume PCR purification Kit
      Low Elution Volume Gel Extraction Kit
      Materials for Agarose Gel Analysis
      Magna Grip™ Rack 8 well (20-400) or similar magnetic rack.
      Instrument capable of quantifying low concentrations of nucleic acids
      References
      Product Information
      Components
      • Magna ChIP A/G (2° to 8°C) -Chromatin IP Reagents
      • Magna ChIP A/G (-20°C) -Chromatin IP Reagents
      • Magna ChIP-Seq Library Construction (-20°C) Reagents
      PresentationThree modules containing all key reagents for chromatin immunoprecipitation and next generation sequencing library preparation from genomic DNA.
      Applications
      ApplicationMagna ChIP-Seq Chromatin IP & Next Generation Sequencing Library Preparation Kit containing all key reagents for chromatin IP & next generation sequencing library preparation from genomic DNA.
      Key Applications
      • ChIP-seq
      • Sample Prep
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceMagna ChIP-Seq Kit Functional Validation:
      ChIP modules are functionally tested in quantitative chromatin immunoprecipitation reactions and each set of library construction reagents is functionally validated together through construction and sequencing of a genomic DNA library on an Illumina Genome Analyzer II.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when used and stored as directed.
      Packaging Information
      Material Size10 library constructions
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      An Introduction to Antibodies and Their Applications
      Magna ChIP-Seq™ Kit
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Technical Info

      Title
      Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      Data Sheet

      Title
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      User Guides

      Title
      Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit