FCABS301A4 | Milli-Mark™ Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 Antibody

FCABS301A4
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue NumberFCABS301A4
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 Antibody
      Alternate Names
      • H3 histone family, member T
      • histone 3, H3
      • histone cluster 3, H3
      Background InformationHistone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • HeLa Cells
      PresentationPurified rabbit polyclonal IgG conjugated to Alexa Fluor 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA
      Applications
      ApplicationMilli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 Antibody detects level of dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenKLH-conjugated, 2X-branched synthetic peptide corresponding to dimethyl-lysine at residue 9 of human Histone H3.
      EpitopeMethylated at Lys9dimethylated Lys 9 of Human Histone H3
      HostRabbit
      SpecificityAntibody recognizes human Histone H3 dimethylated on lysine 9.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Gene Symbol
      • HIST3H3
      • H3FT
      • H3/t
      • H3t
      • MGC126888
      • H3/g
      • H3.4
      • H3T
      • MGC126886
      Modifications
      • Methylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

      SIZE: 136 amino acids; 15508 Da

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Expressed in testicular cells.

      DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity).

      SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.
      Molecular Weight 15.5 kDa Calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor#174;488 - 1966918 1966918
      Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor#174;488 - 2073079 2073079
      Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor#174;488 - 2204961 2204961
      Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor#174;488 - NG1943002 NG1943002
      Milli-Mark Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor#174;488 - NRG1792552 NRG1792552
      Milli-Mark™ Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 -2823955 2823955
      Milli-Mark™ Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 -2887610 2887610
      Milli-Mark™ Anti-dimethyl-Histone H3 (Lys9)-Alexa Fluor®488 Conjugate 2899969
      Milli-Mark™ Anti-dimethyl-Histone H3 -2743626 2743626

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies