FCMAB140P | Milli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18

FCMAB140P
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCRbPhycoerythrinMonoclonal Antibody
      Description
      Catalogue NumberFCMAB140P
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-phospho-Bcl-2 (Ser70) -PE Antibody, clone 69-10C-2-10C-18
      Alternate Names
      • B-cell CLL/lymphoma 2
        B-cell lymphoma protein 2
      Background InformationThe Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl-2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Bcl-2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders. Bcl-2 phosphorylated at Ser-70 plays a critial role in the translocation of Bax from the cytosol to the mitochondria, and this may regulate NO-induced neuronal apoptosis. The molecular mechanism of Bcl-2 phosphorylation and its relationship to Bax is largely unknown.
      References
      Product Information
      FormatPhycoerythrin
      Control
      • Jurkat cells.
      PresentationPurified rabbit monoclonal IgG conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationDetect phospho-Bcl-2 (Ser70) using this Milli-Mark Anti-phospho-Bcl-2 Antibody, clone 69-10C-2-10C-18 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Bcl-2 phosphorylated at Ser70.
      EpitopeSer70
      Clone69-10C-2-10C-18
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes phosphorylated Bcl-2 at Ser70.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq]
      Gene Symbol
      • BCL2
      • Bcl-2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).


      SUBUNIT STRUCTURE: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity By similarity. Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.


      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.


      TISSUE SPECIFICTY: Expressed in a variety of tissues.


      DOMAIN: The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.


      PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) By similarity.


      Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. INVOLVEMENT IN DISEASE: A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.


      SEQUENCE SIMLARITIES: Belongs to the Bcl-2 family.
      Molecular Weight26 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications