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MAB8140-KC | Mouse Anti-Cytomegalovirus Early (1mg) KC

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      Replacement Information
      Catalogue NumberMAB8140-KC
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMouse Anti-Cytomegalovirus Early (1mg) KC
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-CMV
      Product Information
      PresentationPurified ascites. Supplied in PBS buffer, pH 7.4. Contains 0.01% sodium azide and carrier protein. Has been sterile filtered.
      ApplicationAnti-Cytomegalovirus Antibody, early, clone 5A8.2 detects level of Cytomegalovirus & has been published & validated for use in IF, WB, IH & IP.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesImmunochemistry.

      IFA at 1:3,000-1:6,000 on acetone fixed cells.

      Does not work with paraffin embedded tissue sections.

      Dilute with buffer pH 7.4-7.6 to desired working volume.

      For extensive dilution, protein containing or other stabilizing medium should be used.

      Final working dilutions must be determined by end user.
      Biological Information
      ImmunogenAffinity purified antigen from MRC-5 cells infected with CMV AD169 (ATCC).
      SpecificityReacts with Immediate Early 2 protein (IE2) and its fragments. Recognizes a can epitope near the carboxy-end that includes an epitope found in IE2 86, IE2 60 and IE2 40. In western blot recognizes proteins at apparent molecular weights of 86, 68-72, 55 and 38 kD. Can detect CMV infection 1 hour post-infection exhibiting nuclear and/or intranuclear inclusion staining with reaches peak intensity at 3 hours.

      With CMV the antigens expressed at different times are listed as:

      Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

      Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

      Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at +4°C for up to 12 months
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information


      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      MAB8140 Anti-Cytomegalovirus Antibody, early, clone 5A8.2 Show Pricing & Availability

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