SCR513 | Mouse STEMCCA Cre-Excisable Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit

SCR513
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCR513
      DescriptionMouse STEMCCA Cre-Excisable Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit
      OverviewThe Mouse STEMCCA Cre-Excisable Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit is comprised of a cre-excisable, doxycycline (DOX)-inducible polycistronic reprogramming “stem cell cassette” or STEMCCA, which contains the four mouse transcription factors (OKSM) separated by the self-cleaving 2A peptide and IRES7,11-14 sequences. The expression of the four factors are under the control of the doxycycline (DOX)-inducible tetO operator. Co-transfection with a lentivirus constitutively expressing reverse tetracycline transcriptional activator (rtTA) effectively reprogramed somatic cells to an embryonic stem (ES) cell-like state when cells are cultured in the presence of DOX. This single polycistronic cassette supported higher efficiency of reprogramming, reduced the number of viral integrations, enabled shut down of the viral reprogramming transgenes upon the withdrawal of doxycycline and facilitated subsequent Cre-mediated excision of the viral reprogramming transgenes. The results are iPS cells that are virtually free of the exogenous viral trangenes.

      Limited Use Label License:
      This product contains the Tetracycline controllable expression system (the "Tet Technology"). The Tet Technology IP portfolio is proprietary to TET Systems GmbH & Co. KG ("TET Systems") and comprises a series of patents including U.S. Patent Nos. 5,464,758, 5,814,618, and European Patent EP 0804565 and corresponding patent claims outside these regions. The purchaser of this product (Recipient) is granted an automatic license to use the Tet Technology only for internal, academic research purposes, which license specifically excludes the right to sell, or otherwise transfer, the Tet Technology or its component parts to third parties. Recipient whose research using the Tet Technology is sponsored by for-profit organizations, which shall receive ownership to any data or results stemming from the sponsored research, shall need a commercial license agreement from TET Systems in order to use the Tet Technology.
      References
      Product Information
      Components
      • 1. TetO-STEMCCA-LoxP (OKSM) Lentivirus: (Part number CS204442) Two (2) vials, each contains 15 µL of high titer lentiviruses. For exact titer refer to the label on the front of the manual.
      • 2. rtTA Lentivirus: (Part number CS204506) Two (2) vials, each contains 15 µL of high titer constitutively expressed reverse tetracycline transcriptional activator (rtTA) lentiviruses. For exact titer refer to the label on the front of the manual.
      • 3. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.
      Applications
      ApplicationThe Mouse STEMCCA Cre-Excisable Dox-Inducible Polycistronic (OKSM) Lentivirus Reprogramming Kit is comprised of a cre-excisable, doxycycline (DOX)-inducible polycistronic reprogramming “stem cell cassette” or STEMCCA.
      Biological Information
      Stem Cell Type
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested to confirm the generation of iPS cells from p3 mouse embryonic fibroblasts. Cre-mediated exicsion of the reprogramming transgenes has been validated in the mouse system. Other cell types have not been tested and thus similar results can not be guaranteed.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications