96-well strip plate  
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      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      Catalogue NumberEZMTNFA
      DescriptionMouse TNF-α ELISA Kit, EZMTNFA
      OverviewThe Mouse TNF-α ELISA Kit is a Sandwich Enzyme- Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre- coated with a capture antibody. This kit is specifically designed for the accurate quantitation of mouse TNF-α from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
      Background InformationMouse TNF-α (Tumor Necrosis Factor α) is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (primarily CD4+ T cells), NK- cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing. The membrane form protein, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and pathological conditions, including neurodegenerative diseases and cancer..
      Product Information
      Precision, %
      • Intra-assay: <10 %
      • Inter-assay: <15 %
      ApplicationThis Mouse TNF-α ELISA Kit, EZMTNFA is used to measure & quantify TNF-α levels in Metabolism & Neuroscience research.
      Key Applications
      • ELISA
      Biological Information
      SpecificityNo cross reactivity was observed when this kit was used to analyze multiple human, mouse and rat recombinant proteins other than mouse TNF-α.
      Species Reactivity
      • Mouse
      Analytes Available
      • TNF-α
      • 111%
      Physicochemical Information
      • 1.5 pg/mL
      Linearity of DilutionContact Technical Service for this information
      Standard Curve Range
      • 15.6-1000 pg/mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 


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      96 well Template 2 Up
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      Mouse TNF-α ELISA Kit

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      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits