S7450 | OxyIHC™ Oxidative Stress Detection Kit

S7450
50 Reactions  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      IHCChromogenic
      Description
      Catalogue NumberS7450
      Trade Name
      • OxyIHC
      DescriptionOxyIHC™ Oxidative Stress Detection Kit
      OverviewThe OxyIHC™ Oxidative Stress Detection Kit (S7450) contains the chemical and immunological reagents
      necessary to detect protein oxidation in various tissues from a variety of organs and animal species. The
      OxyIHC test method involves chemical derivatization of protein carbonyl groups with 2,4-
      dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNP
      at their carbonyl sites. The DNP-derivatized proteins are then detected using an antibody that specifically
      binds to the DNP moiety. Subsequent incubation with biotin conjugated secondary antibody, streptavidin
      conjugated HRP, and development using a 3,3’ diaminobenzidine (DAB) staining allows
      immunohistochemical detection of protein oxidation.
      Background InformationReactive oxygen species (ROS) have been implicated in pathological processes including cancer,
      apoptosis, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and
      cardiovascular diseases (for reviews, see ref. 1-4). Living organisms are continually exposed to potentially
      harmful oxygen free radicals that are generated thru normal cellular functions as well as from environmental
      factors (1, 5-7). Yet, these reactive molecules are mitigated by the presence of antioxidants and therefore
      organisms exist with a delicate balance between oxidants and antioxidants (7-9). During times of oxidative
      stress there is an imbalance in favor of the oxidants. The end result of which is that free radicals are able to
      attack and modify sub-cellular components including nucleic acids, lipids and proteins (7, 10-12).
      Proteins are one of the major targets of reactive species. Oxidation of proteins modifies the side chains of
      methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of
      proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine
      residues in a site-specific manner (16, 20-22). The oxidative modification of proteins can modulate
      biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of
      transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a
      relationship between protein oxidation and aging has been suggested (29-31), little is known about the
      importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated
      processes. Not surprisingly, carbonyl modification of proteins has become a key biomarker for the
      identification of oxidative stress.
      References
      Product Information
      Components
      • 10X Wash Buffer, One 25 mL bottle
      • 10X Antigen Retrieval Buffer, One 1.25 mL bottle
      • 5X Blocking Buffer, One 2.5 mL bottle
      • Primary Antibody, One 125 mL vial
      • DAB-A, One 500 mL vial
      • DAB-B, One 12 mL bottle
      • Antibody Diluent, One ready to use 12.5 mL bottle
      • Biotinylated Secondary Antibody, One ready to use 12.5 mL bottle
      • Streptavidin conjugated HRP, One ready to use 12.5 mL bottle
      • Hematoxylin, One ready to use 12.5 mL bottle
      • DNPH Solution, One ready to use 12.5 mL bottle
      • Derivatization Control Solution, One ready to use 12.5 mL bottle
      Detection methodChromogenic
      Applications
      ApplicationThe OxyIHC Oxidative Stress Detection Kit contains the chemical & immunological reagents necessary to detect protein oxidation in various tissues from a variety of organs & animal species.
      Key Applications
      • Immunohistochemistry
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe OxyIHC kit components should be stored at 2-8ºC. Kit components are stable for 6 months from the
      date of receipt if stored and handled properly.
      Packaging Information
      Material Size50 Reactions
      Transport Information
      Supplemental Information
      Specifications