ST1013 | PhosphoDetect™ Anti-GSK-3α/β (pTyr279/216) Rabbit pAb

ST1013
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RRbPolyclonal Antibody

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      ST1013-10T
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      In Stock 
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~51 kDa GSK-3α protein phosphorylated at Tyr279 and the ~47 kDa protein GSK-3β phosphorylated at Tyr216.
          Note: 1T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue NumberST1013
          Brand Family Calbiochem®
          SynonymsAnti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)
          Application Data
          Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
          References
          ReferencesPapasozomenos, S.C., et al. 2002. PNAS 99, 1140.
          Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
          Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
          Negative controlNormal rabbit serum
          Positive controlSerum starved insulin-treated 3T3-L1 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Dot Blot
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesDot Blot (see comments)
          ELISA (see coments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:400)
          Application CommentsTo generate a positive control, serum starve 3T3-L1 cells for 3 h, then stimulate with 100 nM insulin or 100 ng/ml IGF-1 for 5 min. This antibody has also been reported to work for ELISA and dot blots. At a 1:1000 dilution there is enough antibody for 10 immunoblots at 10 ml/blot. Antibody must be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          ST1013

          References

          Reference overview
          Papasozomenos, S.C., et al. 2002. PNAS 99, 1140.
          Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
          Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-May-2008 JSW
          SynonymsAnti-Glycogen Synthase Kinase-3α/β, Phospho-Specific (Tyr279/216)
          ApplicationDot Blot (see comments)
          ELISA (see coments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:400)
          Application Data
          Detection of human GSK-3β, phosphorylated at Tyr216 by immunoblotting. Samples: Whole cell lysate from control cells (lane 1) or control lysate spiked with human recombinant GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Glycogen Synthase Kinase-3α/β (pTyr279/216) Rabbit pAb (Cat. No. ST1013) (1:1000) pre-incubated without peptide (lanes 1, 5), or with phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~51 kDa phosphorylated GSK-3α protein phosphorylated at Ser279 and the ~47 kDa GSK-3β protein phosphorylated at Ser216.
          BackgroundGSK-3α contains a phosphate on tyrosine 279, and GSK-3β contains a phosphate on tyrosine 216. GSK is a protein serine kinase involved in the control of regulatory proteins like glycogen synthase and the transcription factor c-Jun. It has also been associated with the regulation of the microtubule-binding protein Tau, and may be involved in the pathogenesis of Alzheimer's Disease.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr279/216 phosphorylation sites of human GSK-3α/β
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlSerum starved insulin-treated 3T3-L1 cells
          Negative controlNormal rabbit serum
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 50% glycerol, 1 mg/ml BSA, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsTo generate a positive control, serum starve 3T3-L1 cells for 3 h, then stimulate with 100 nM insulin or 100 ng/ml IGF-1 for 5 min. This antibody has also been reported to work for ELISA and dot blots. At a 1:1000 dilution there is enough antibody for 10 immunoblots at 10 ml/blot. Antibody must be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesPapasozomenos, S.C., et al. 2002. PNAS 99, 1140.
          Kirschenbaum, F., et al. 2001. J. Biol. Chem. 276, 7366.
          Bhat, R.V., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 11074.

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