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16-157 Protein A Agarose/Salmon Sperm DNA, 2.5 mL

16-157
2.5 mL  2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
Purchase on Sigma-Aldrich

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Achieve first time ChIP success with EZ-ChIP Assays
Description
Catalogue Number16-157
Brand Family Upstate
Trade Name
  • Upstate
DescriptionProtein A Agarose/Salmon Sperm DNA, 2.5 mL
OverviewProtein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Alternate Names
  • ChIP agarose beads
  • ChIP agaraose A beads
Background InformationProtein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
References
Product Information
Presentation10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
Applications
ApplicationRecombinant protein A covalently bound to agarose beads for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H4 and H3 ChIP assay.
Application NotesSuitable for use in chromatin immunoprecipitation (ChIPs) assays
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at 4°C
Packaging Information
Material Size2.5 mL
Material Package2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
Transport Information
Supplemental Information
Specifications

Documentation

Protein A Agarose/Salmon Sperm DNA, 2.5 mL SDS

Title

Safety Data Sheet (SDS) 

Protein A Agarose/Salmon Sperm DNA, 2.5 mL Certificates of Analysis

TitleLot Number
Protein A agarose/Salmon Sperm DNA - 06070344700607034470
Protein A agarose/Salmon Sperm DNA - 06080381930608038193
Protein A agarose/Salmon Sperm DNA - 07010493850701049385
Protein A agarose/Salmon Sperm DNA - 1712417124
Protein A agarose/Salmon Sperm DNA - 1811018110
Protein A agarose/Salmon Sperm DNA - 1916019160
Protein A agarose/Salmon Sperm DNA - 1962019620
Protein A agarose/Salmon Sperm DNA - 2077120771
Protein A agarose/Salmon Sperm DNA - 2115121151
Protein A agarose/Salmon Sperm DNA - 2189821898

References

Reference overviewApplicationPub Med ID
A novel HSI2 mutation in Arabidopsis affects the PHD-like domain and leads to derepression of seed-specific gene expression.
Vijaykumar Veerappan,Jing Wang,Miyoung Kang,Joohyun Lee,Yuhong Tang,Ajay K Jha,Huazhong Shi,Ravishankar Palanivelu,Randy D Allen
Planta  236  2012

Show Abstract
22476218 22476218
The adenovirus L4-22K protein is multifunctional and is an integral component of crucial aspects of infection.
Wu, Kai, et al.
J. Virol., 86: 10474-83 (2012)  2012

Show Abstract
22811519 22811519
Methods for analyzing histone citrullination in chromatin structure and gene regulation.
Pingxin Li,Jing Hu,Yanming Wang
Methods in molecular biology (Clifton, N.J.)  809  2012

Show Abstract
22113295 22113295
In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population.
Foster JG, Cassida KA, Turner KE
Vet Parasitol  2011

Show Abstract
21477927 21477927
The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma.
Matthew Bott,Marie Brevet,Barry S Taylor,Shigeki Shimizu,Tatsuo Ito,Lu Wang,Jenette Creaney,Richard A Lake,Maureen F Zakowski,Boris Reva,Chris Sander,Robert Delsite,Simon Powell,Qin Zhou,Ronglai Shen,Adam Olshen,Valerie Rusch,Marc Ladanyi
Nature genetics  43  2011

Show Abstract
21642991 21642991
A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.
Du, JX; McConnell, BB; Yang, VW
The Journal of biological chemistry  285  28298-308  2010

Show Abstract
20584900 20584900
Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
Lauren J Buro,Shaili Shah,Melissa A Henriksen
Journal of visualized experiments : JoVE  2010

Show Abstract
20729799 20729799
Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications.
Yao H, Hwang JW, Moscat J, Diaz-Meco MT, Leitges M, Kishore N, Li X, Rahman I
The Journal of biological chemistry  285  5405-16  2010

Show Abstract Full Text Article
20007975 20007975
The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation.
Ralf Stracke,Jean-Jacques Favory,Henriette Gruber,Lutz Bartelniewoehner,Sebastian Bartels,Melanie Binkert,Markus Funk,Bernd Weisshaar,Roman Ulm
Plant, cell & environment  33  2010

Show Abstract
19895401 19895401
Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo.
Monica E Ferreira, Philippe Prochasson, Kurt D Berndt, Jerry L Workman, Anthony P H Wright
The FEBS journal  276  2557-65  2009

Show Abstract
19476494 19476494

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Title
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Title
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