ECM508 | QCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric

ECM508
1 plate  24 wells
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsDetection Methods
      ACTChromogenic
      Description
      Catalogue NumberECM508
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM Chemotaxis Cell Migration Assay, 24-well (8 µm), colorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation. Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. Recently, a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell migration. The effect of this treatment on cell behavior/migration remains questionable.

      The CHEMICON® QCM™ 24-well Cell Migration Assay (ECM508) eliminates cell pre-labeling and manual counting. The 24-well insert and colorimetric detection format allows for quantitative comparison of multiple samples.

      In the CHEMICON® QCM™ 24-well Migration Assay, cells that have migrated to the bottom of the insert membrane are stained. The stain is then extracted and transferred to a 96-well microtiter plate for colorimetric measurement.

      The CHEMICON® QCM™ 24-well Migration Assay provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.

      The CHEMICON® QCM™ 24-well Migration Assay utilizes an 8 mm pore size, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.



      In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:

      · QCM™ 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)

      · QCM™ 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)

      · QCM™ 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)

      · QCM™ 96-well Cell Invasion Assay (ECM555)

      · QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)

      · 24-well Insert Cell Migration and Invasion Assay Systems

      · CytoMatrix™ Cell Adhesion strips (ECM protein coated)

      · QuantiMatrix™ ECM protein ELISA kits



      Test Principle

      The CHEMICON® QCM™ 24-well Cell Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. Each kit contains 24 inserts; each insert utilizes an 8 mm pore size polycarbonate membrane, as this is appropriate for most cell types. This pore size supports optimal migration for most epithelial and fibroblast cells; however, it is not appropriate for lymphocyte migration experiments. Cells that have migrated through the polycarbonate membrane are incubated with Cell Stain Solution, then subsequently extracted and detected on a standard microplate reader (560 nm). The system may be adapted to study different types of cell migration, including haptotaxis, random migration, chemokinesis, and chemotaxis.
      Materials Required but Not Delivered1. Precision pipettes: sufficient for aliquoting cells.

      2. Harvesting buffer: EDTA or trypsin cell detachment buffer. Suggested formulations include a) 2 mM EDTA/PBS, b) 0.05% trypsin in Hanks Balanced Salt Solution (HBSS) containing 25 mM HEPES, or other cell detachment formulations as optimized by individual investigators.

      Note: Trypsin cell detachment buffer maybe required for difficult cell lines. Allow sufficient time for cell receptor recovery.

      3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 10% FBS.

      4. Chemoattractants (eg. 10% FBS) or pharmacological agents for addition to culture medium, if screening is desired.

      5. Quenching Medium: serum-free medium, such as DMEM, EMEM, or FBM (fibroblast basal media), containing 5% BSA.

      Note: Quenching Medium must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in the harvesting buffer.

      6. Sterile PBS or HBSS to wash cells.

      7. Distilled water.

      8. Low speed centrifuge and tubes for cell harvesting.

      9. CO2 incubator appropriate for subject cells.

      10. Hemocytometer or other means of counting cells.

      11. Trypan blue or equivalent viability stain.

      12. Microplate reader (560 nm).

      13. 24-well tissue culture plate.

      14. Sterile cell culture hood.
      References
      Product Information
      Components
      • Sterile 24-well Cell Migration Plate Assembly: (Part No. 90333) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
      • Cell Stain: (Part No. 90144) One 20 mL bottle.
      • Extraction Buffer: (Part No. 90145) One 20 mL bottle.
      • Cotton Swabs: (Part No. 10202) 50 each.
      • Forceps: (Part No. 10203) One each.
      Detection methodChromogenic
      Applications
      ApplicationThe QCM 24-well Migration Assay is ideal for the study of chemotaxis cell migration. The assay uses a 24-well plate with an 8 micron pore size, with colorimetric detection.
      Key Applications
      • Activity Assay
      Application NotesThe CHEMICON® QCM™ 24-well Migration Assay is ideal for the study of chemotaxis cell migration. Each Chemicon Cell Migration Assay Kit contains sufficient reagents for the evaluation of 24 samples. The quantitative nature of this assay is especially useful for screening of pharmacological agents.

      The CHEMICON® QCM™ 24-well Migration Assay is intended for research use only; not for diagnostic applications.
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at 2-8°C for up to their expiration date. Do not freeze.
      Packaging Information
      Material Size1 plate
      Material Package24 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Plasma rich in growth factors (PRGF-Endoret) stimulates proliferation and migration of primary keratocytes and conjunctival fibroblasts and inhibits and reverts style=background-c
      Anitua E, Sanchez M, Merayo-Lloves J, De la Fuente M, Muruzabal F, Orive G
      Investigative ophthalmology & visual science  52  6066-73. Print 2011 Aug.  2011

      21613374 21613374
      The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration.
      Keith R Anderson,Ruth A Singer,Dina A Balderes,Laura Hernandez-Lagunas,Christopher W Johnson,Kristin B Artinger,Lori Sussel
      Development (Cambridge, England)  138  2011

      Show Abstract
      21750032 21750032
      Substance P Modulates Chronic Inflammation-Induced Colonic Fibrosis.
      Koon HW, Shih D, Karagiannides I, Zhao D, Fazelbhoy Z, Hing T, Xu H, Lu B, Gerard N, Pothoulakis C
      Am J Pathol  2010

      Show Abstract
      20889569 20889569
      Presenilin 1/gamma-secretase is associated with cadmium-induced E-cadherin cleavage and COX-2 gene expression in T47D breast cancer cells.
      Chang Seok Park,Ohn Soon Kim,Sang-Moon Yun,Sangmee A Jo,Inho Jo,Young Ho Koh
      Toxicological sciences : an official journal of the Society of Toxicology  106  2008

      Show Abstract
      18791180 18791180

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      User Guides

      Title
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      Categories

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