HTS178LRTA | Ready-to-Assay™ FSH Glycoprotein Hormone Receptor Frozen Cells

HTS178LRTA
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      Overview

      Replacement Information

      Special Offers

      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Spec Table

      SpeciesHost CellsProtein Target
      HumanHEK293FSH
      Description
      Catalogue NumberHTS178LRTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ FSH Glycoprotein Hormone Receptor Frozen Cells
      Background InformationEMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      EMD Millipore’s ChemiBrite cells co-express a GPCR along with a novel variant of clytin, a calcium-activated photoprotein, to enable sensitive luminescent detection of ligand-induced calcium flux. The ChemiBrite version of clytin contains a mutation that increases its affinity for calcium to a level that permits detection of cytosolic calcium in many cells with greater sensitivity than other mitochondrially expressed photoproteins. Luminescent calcium assays offer several advantages over fluorescent calcium assays including; lower substrate cost, increased sensitivity, and lack of interference from fluorescent compounds.

      Follicular Stimulating Hormone (FSH) is a critical hormone in mammalian reproduction. In concert with Luteinizing Hormone (LH), this gonadotropin is secreted by the pituitary gland as a glycoprotein and affects all aspects of fertility and reproduction. In females, FSH drives ovarian development, maturation and cycle regulation. In males, FSH mediates spermatid maturation. FSH receptors couple to Gαs thus utilizing cAMP production to mediate cellular effects (Verrier et al). EMD Millipore’s cloned human FSH receptor-expressing ChemiBrite cells were made by stable transfection of HEK293 cells with ChemiBrite clytin, the FSH receptor (FSHR) and a promiscuous G protein to couple the receptor to the calcium signaling pathway, then cryopreserving the cells at an optimal time post-transfection. Upon thaw, recovery, and loading, the cells are ready for luminescent, fluorescent, and cAMP analysis of agonists, antagonists and modulators at the FSH receptor.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Applications
      Key Applications
      • Calcium Flux Assays
      • cAMP Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsHEK293
      Protein TargetFSH
      Target Sub-FamilyGlycoprotein hormone
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      The follicle-stimulating hormone receptor: biochemistry, molecular biology, physiology, and pathophysiology.
      Simoni, M, et al.
      Endocr. Rev., 18: 739-73 (1997)  1997

      9408742 9408742
      Thyrotropin binding to and adenylate cyclase activity of porcine thyroid plasma membranes.
      Verrier, B, et al.
      Eur. J. Biochem., 74: 243-52 (1977)  1977

      Show Abstract
      192550 192550

      Data Sheet

      Title
      Ready-to-Assay™ FSH Glycoprotein Hormone Receptor Frozen Cells

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells