HTS176RTA | Ready-to-Assay™ S1P1 Lysophospholipid Receptor Frozen Cells

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      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Spec Table

      SpeciesHost CellsProtein Target
      HumanChem-4S1P1 / EDG1
      Catalogue NumberHTS176RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ S1P1 Lysophospholipid Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      Sphingosine 1-phosphate (S1P) is a biologically active lysophospholipid that transmits signals through a family of five G-protein-coupled receptors to regulate cell proliferation, migration, cytoskeletal organization, and differentiation. S1P1 was the first identified S1P receptor. It primarily couples to PTX-sensitive Gi/o proteins and mediates S1P-induced adenylate cyclase inhibition. Expression of S1P1 is pervasive, including spleen, brain, heart, lung, adipose tissues, liver, thymus, kidney, and skeletal muscle (Zhang et al. 1999). The deletion of S1P1 in mice results in embryonic lethality (Liu et al., 2000) with death attributable to incomplete vascular maturation. Recent reports demonstrate specific roles for S1P1 in lymphocyte recirculation/egress (Matloubian et al., 2004). Chemicon's cloned human S1P1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P1 and its ligands.
      Product Information
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      ApplicationHuman S1P1 / EDG1 GPCR Frozen Cells for calcium flux FLIPR Assays.
      Key Applications
      • Ligand Binding Assays
      Biological Information
      GPCR ClassA
      Host CellsChem-4
      Protein TargetS1P1 / EDG1
      Target Sub-FamilyLysophospholipid
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Sales RestrictionsThis product requires a User Agreement (Label License). Please see product datasheet for further information.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPlace cells in liquid nitrogen immediately upon receipt.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information

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      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells