03-0052-00 | Singulex Erenna SMC Human Interleukin 4 (IL-4)
VERIFIED IMMUNOASSAY KIT

03-0052-00
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-4HSingle Molecule Counting
      Description
      Catalogue Number03-0052-00
      Trade Name
      • Singulex
      DescriptionSingulex Erenna SMC Human Interleukin 4 (IL-4)
      VERIFIED IMMUNOASSAY KIT
      OverviewThe Singulex® Erenna® SMC® Human IL-4 Immunoassay Kit contains all reagents required to perform assays on the Erenna® Immunoassay System. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® Immunoassay System detects analytes using Single Molecule Counting (SMC®) technology.
      Background InformationInterleukin-4 (IL-4), is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets, and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
      References
      Product Information
      Components
      • IL-4 Coated Beads
      • Standard Diluent
      • IL-4 Detection Antibody
      • Assay Buffer
      • IL-4 Standard
      • Erenna® Human IL-4 Immunoassay Kit Instructions
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      Precision, %
      • Low-end CV% Range: 2 - 12%
      • Low-end CV% Average: 7%
      Applications
      ApplicationThe sensitivity of Single Molecule Counting (SMC) from Singulex gives the Erenna SMC Human IL-4 Immunoassay Kit has unparalleled performance in the quantification of IL-4 in human EDTA plasma.
      Key Applications
      • Single Molecule Counting
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-4
      Accuracy
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Erenna SMC™ Human IL-4 Immunoassay Kit Instructions

      SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      Protein Detection Platform and Services Brochure
      Singulex Erenna® Immunoassay System

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods

      Posters

      Title
      Multiplex Assays of Fifteen Human Complement Factors