114666 Actinomycin D, Streptomyces sp. - CAS 50-76-0 - Calbiochem

Actinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I

Primary Target: serine proteases
114666
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      Overview

      Replacement Information

      Key Spec Table

      Empirical FormulaCAS #
      C₆₂H₈₆N₁₂O₁₆50-76-0

      Pricing & Availability

      Catalogue NumberAvailability Packaging Qty/Pack Price Quantity
      114666-1SET
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          		 Fibre case 1 set
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          114666-5MG
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              		 Plastic Bag(s) 5 mg
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              Description
              OverviewAnti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. At higher concentrations, DNA polymerase is inhibited. Also acts as a competitive inhibitor of serine proteases. Inhibits cell growth and colony formation in synchronized HeLa cells. Induces apoptosis in many cell lines. However, actinomycin D has also been shown to suppress etoposide-induced apoptosis in PC12 cells. Note: 1 set = 20 x 200 µg.
              Stability: heat resistant and stable between pH 2 and 11. Decomposed by strong acids and bases.
              Catalogue Number114666
              Brand Family Calbiochem®
              SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
              References
              ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
              Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
              Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
              Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
              Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
              Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
              White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
              Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
              Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
              Product Information
              CAS number50-76-0
              FormRed crystalline solid
              Hill FormulaC₆₂H₈₆N₁₂O₁₆
              Chemical formulaC₆₂H₈₆N₁₂O₁₆
              Hygroscopic Hygroscopic
              Structure formula ImageStructure formula Image
              Applications
              ApplicationActinomycin D, Streptomyces sp., CAS 50-76-0, is an anti-neoplastic antibiotic that inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.
              Biological Information
              Primary Targetserine proteases
              Secondary targetcell growth and colony formation in synchronized HeLa cells
              Purity≥98% by HPLC
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSAU1575000
              Safety Information
              R PhraseR: 28

              Very toxic if swallowed.
              S PhraseS: 28-36/37-45


              Wear suitable protective clothing and gloves.
              In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Highly Toxic & Carcinogenic / Teratogenic
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage +2°C to +8°C
              Protect from Light Protect from light
              Hygroscopic Hygroscopic
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              114666

              References

              Reference overview
              Nakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
              Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
              Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
              Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
              Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
              Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
              White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
              Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
              Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.

              Brochure

              Title
              Caspases and other Apoptosis Related Tools Brochure
              Nitric Oxide and Oxidative Stress Brochure
              Tools and Tips for Analyzing Apoptosis

              Citations

              Title
            • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
            • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
            • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
              		•
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision07-August-2009 JSW
              SynonymsDactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
              DescriptionAntineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.
              FormRed crystalline solid
              CAS number50-76-0
              RTECSAU1575000
              Chemical formulaC₆₂H₈₆N₁₂O₁₆
              Structure formulaStructure formula
              Purity≥98% by HPLC
              SolubilityDMSO (1 mg/ml), Methanol, or Chloroform
              Storage +2°C to +8°C
              Hygroscopic
              Protect from light
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
              Toxicity Highly Toxic & Carcinogenic / Teratogenic
              Merck USA index14, 2800
              ReferencesNakajima, M., et al. 1994. Neurosci. Lett. 176, 161.
              Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
              Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
              Yung, B.Y., et al. 1992. Int. J. Cancer 52, 317.
              Martin, S.J., et al. 1990. J. Immunol. 145, 1859.
              Yung, B.Y., et al. 1990. Cancer Res. 50, 5987.
              White, R.J., and Phillips, D.R. 1985. Biochemistry 27, 9122.
              Madharavao, M., et al. 1978. J. Med. Chem. 21, 958.
              Sengupta, S.K., et al. 1975. J. Med. Chem. 18, 1175.
              Citation
            • Alexandra Thiel, Mira Heinonen, Johanna Rintahaka, Tuija Hallikainen, and Annabrita Hemmes, Dan A. Dixon, Caj Haglund, and Ari Ristimaki. (2006) EXPRESSION OF CYCLOOXYGENASE-2 IS REGULATED BY GSK-3 IN GASTRIC CANCER CELLS. Journal of Biological Chemistry in press,.
            • Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
            • Alexandra Thiel, et al. (2006) Expression of Cyclooxygenase-2 Is Regulated by Glycogen Synthase Kinase-3beta in Gastric Cancer Cells. Journal of Biological Chemistry 281, 4564-4569.
              		•