Total Drug Analysis for Pharmacokinetic Studies

MultiScreen® Deep Well and MultiScreen Solvinert Filter Plates

Overview

Specifications

Ordering Information

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MultiScreen Deep Well Solvinert Filter Plates Clear Sorting & Filtering
Catalogue Numbericon Chemistryicon Pack Sizeicon
MDRLN0410Hydrophilic Polytetrafluoroethylene (PTFE) 10 Show Pricing & Availability
MDRPN0410Hydrophobic Polytetrafluoroethylene (PTFE) 10 Show Pricing & Availability

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MultiScreen Deep Well Solvinert Filter Plates with prefilter Clear Sorting & Filtering
Catalogue Numbericon Chemistryicon Pack Sizeicon
MDRPNP410Hydrophobic Polytetrafluoroethylene (PTFE) 10 Show Pricing & Availability

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MultiScreen Solvinert Filter Plates Clear Sorting & Filtering
Catalogue Numbericon Chemistryicon Pack Sizeicon
MSRLN0410Hydrophilic Polytetrafluoroethylene (PTFE) 10 Show Pricing & Availability
MSRLN0450Hydrophilic Polytetrafluoroethylene (PTFE) 50 Show Pricing & Availability
MSRPN0410Hydrophobic Polytetrafluoroethylene (PTFE) 10 Show Pricing & Availability
MSRPN0450Hydrophobic Polytetrafluoroethylene (PTFE) 50 Show Pricing & Availability

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Accessories Clear Sorting & Filtering
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MSVMHTS00MultiScreenHTS Vacuum Manifold 1 Show Pricing & Availability
MSVMHTS0DDeep Well Collar 1 Show Pricing & Availability
MSVMHTS0HCollar Holder, for automation 1 Show Pricing & Availability

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    Documentation

    Technical Info

    Title
    High throughput sample preparation for the quantitation of drug compounds in serum samples
    MultiScreen Deep Well Solvinert Filter Plate: High throughput sample preparation for the quantitation of drug compounds in serum samples

    Data Sheet

    Title
    MultiScreen Solvinert and MultiScreen Deep Well Solvinert Filter Plates; 96-well chemically compatible sample prep platforms

    Posters

    Title
    Automated Sample Preparation Method for High Throughput Total Drug Analysis by LC-MS/MS
    Automation of ADME Applications
    Poster: Rapid Sample Preparation Method for High Throughput Total Drug Analysis by LC-MS/MS

    References

    Reference overviewApplication
    LC–MS-MS Total Drug Analysis of Biological Samples Using a High-Throughput Protein Precipitation Method
    Bogdan Sleczka, Jian Wang, and Timothy Olah, Bristol-Myers Squibb Bioanalytical and Discovery Analytical Sciences, Princeton, New Jersey
    LC-GC, Volume 24 Number 7 July 2006  2006

    Assessment of filter plates for multi-well in-gel digestion of proteins separated by polyacrylamide gel elelctrophoresis to identify them with LC-ESI/MSMS
    Kazuhisa Kameyama, Tomohiro Nanri, Yuko Yamanaka, Mikikio Arima, Hiroshi Kawasaki
    Journal Electrophoresis. 2005; 49, 71-75  2005

    Mass Spectrometry

    FAQ

    QuestionAnswer
    What is the maximum volume that should be used with the MultiScreen Deep Well filter plate?The maximum recommended volume to be used with the MultiScreen Deep Well filter plate is 1.8 mL (1.5 mL with shaking).
    Should the MultiScreen Solvinert Deep Well plate be used with vacuum or centrifuge?The MultiScreen Solvinert Deep Well plate with both vacuum and centrifuge. However, vacuum should not be used with volitile solvents (e.g., TFA).
    At what g force should the MultiScreen Solvinert Deep Well plates be spun?The MultiScreen Solvinert Deep Well plates should be spun at 500-3,000 x g.
    What is the active membrane surface area of the MultiScreen Solvinert Deep Well plate?The active membrane surface area of the MultiScreen Solvinert Deep Well plate is 0.28cm2.
    What is the extractables level for the MultiScreen Solvinert Deep Well filter plate?The extractables level of the MultiScreen Solvinert Deep Well plates is extremely low, making this product ideal for processing samples to be analyzed by HPLC or LC/MS/MS.
    When I wet out the membranes in the MultiScreen Solvinert Deep Well plate they become translucent. Is this normal?Yes, this is completely normal and expected.
    Is the MultiScreen Solvinert Deep Well plate automation compatible?Yes, the MultiScreen Solvinert Deep Well plate is automation compatible.
    When using the MultiScreen Solvinert or Solvinert Deep Well for Total Drug Analysis, can I add the serum all at once to each well? Why do I have to drip the serum in slowly for the manual procedure?Slow addition of the serum to each well allows for maximum surface area exposure of the plasma with the acetonitrile. Subsequent mixing of the solution provides for complete dispersion of the plasma into the acetonitrile thus allowing complete protein precipitation.
    When using the MultiScreen Solvinert or Solvinert Deep Well for Total Drug Analysis, why does the protocol require 90% acetonitrile? Can I use 100% acetonitrile?The specific density of acetonitrile and plasma are rather different, which can lead to incomplete mixing. Prewetting of the acetonitriile with 10% water improves mixing and allows for complete integration of the plasma/serum into the acetonitrile.
    When using the MultiScreen Solvinert or Solvinert Deep Well plate for Total Drug Analysis, how fast should I shake the plate after adding the serum/plasma and acetonitrile to each well? At what setting should my plate shaker be set?It is recommended to shake the plate as fast as possible without spilling the solution from the wells. With the clear wells of the Solvinert Deep Well plate it is easy to see the level of the solution in the wells as it shakes. Simply turn the dial of the plate shaker up until the solution is approaching the top of the wells. It has been our experience that plate shakers can have different gauge settings, and even the same setting on different plates can have different actual rates.

    User Guides

    Title
    MultiScreen Deep Well Solvinert Filter Plate User Guide

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    Categories

    Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates