AB3098 Anti-Orexin-A Antibody

AB3098
50 µg  
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      Tabela kluczowych gatunków

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Fe ELISA, WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB3098
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Orexin-A Antibody
      References
      Product Information
      FormatAffinity Purified
      PresentationLiquid in PBS, pH 7.4 with 0.1% BSA and 0.1% sodium azide.
      Applications
      ApplicationThis Anti-Orexin-A Antibody is validated for use in ELISA, WB for the detection of Orexin-A.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using Chemiluminescence technique ELISA: 1:10,000 - 100,000 using 5-100 ng of control peptide per well. NOTE: Orexin A is a 3.5kDa protein. Adjust your SDS-PAGE gel and lane size markers accordingly.

      Immunohistochemistry: Frozen 4% PFA tissue with ABC detection {Wagner et al 2000}.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenAn 18 amino acid peptide mapping near the amino terminus of mouse Orexin-A. The immunogen sequence is identical in human, rat, porcine, ovine, bovine and canine and has 81% homology with chicken.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityMouse Orexin-A. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Feline
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a hypothalamic neuropeptide precusor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
      Gene Symbol
      • HCRT
      • Hypocretin
      • OX
      • orexin
      • Hcrt
      • PPOX
      • PPORX
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43612 # Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
      SIZE: 131 amino acids; 13363 Da
      SUBCELLULAR LOCATION: Rough endoplasmic reticulum (By similarity). Cytoplasmic vesicle (By similarity). Cell junction, synapse (By similarity). Note=Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses (By similarity).
      TISSUE SPECIFICITY: Abundantly expressed in subthalamic nucleus but undetectable in other brain regions tested (hypothalamus was not tested) and in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides.
      DISEASE: SwissProt: O43612 # Defects in HCRT are a cause of narcolepsy [MIM:161400]. Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed.
      SIMILARITY: SwissProt: O43612 ## Belongs to the orexin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-OREXIN-A - 29928392992839
      RABBIT ANTI-OREXIN-A -27863522786352
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY2897762
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY3051514
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY3101577
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY - 24363152436315
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY - 20060332006033
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY - 20513392051339
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY - 22808382280838
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY -27339192733919

      References

      Reference overviewApplicationPub Med ID
      Hypothalamus-olfactory system crosstalk: orexin a immunostaining in mice.
      Gascuel, J; Lemoine, A; Rigault, C; Datiche, F; Benani, A; Penicaud, L; Lopez-Mascaraque, L
      Frontiers in neuroanatomy  6  44  2012

      Pokaż streszczenie
      Immunohistochemistry23162437 23162437
      Organization and number of orexinergic neurons in the hypothalamus of two species of Cetartiodactyla: a comparison of giraffe (Giraffa camelopardalis) and harbour porpoise (Phocoena phocoena).
      Dell, LA; Patzke, N; Bhagwandin, A; Bux, F; Fuxe, K; Barber, G; Siegel, JM; Manger, PR
      Journal of chemical neuroanatomy  44  98-109  2012

      Pokaż streszczenie
      22683547 22683547
      Immunohistochemical evidence of Orexin-A in the pancreatic beta cells of domestic animals.
      C Dall'Aglio,V Pedini,P Scocco,C Boiti,P Ceccarelli
      Research in veterinary science  89  2010

      Pokaż streszczenie
      20359730 20359730
      Immunohistochemical identification and localization of orexin A and orexin type 2 receptor in the horse gastrointestinal tract.
      Cecilia Dall'aglio,Luisa Pascucci,Francesca Mercati,Andrea Giontella,Vera Pedini,Piero Ceccarelli
      Research in veterinary science  86  2009

      Pokaż streszczenie
      18707745 18707745

      Data Sheet

      Title
      RABBIT ANTI-OREXIN-A POLYCLONAL ANTIBODY

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      Kategorie

      Life Science Research > Antibodies and Assays > Primary Antibodies