2752 BrdU Cell Proliferation Kit

2752
1000 assays  
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      Key ApplicationsDetection Methods
      ELISA Colorimetric
      Description
      Catalogue Number2752
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionBrdU Cell Proliferation Kit
      OverviewThe CHEMICON BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.

      Test Principle

      Within CHEMICON's BrdU Cell Proliferation Assay Kit, mouse monoclonal antibody is used to detect the BrdU in a sample. After addition of goat anti-mouse IgG-peroxidase conjugated secondary antibody, substrate and stop solution, the amount of BrdU is determined. The higher the OD, the higher the BrdU concentration in the sample.



      For Research Use Only. Not for use in diagnostic procedures.
      Background InformationEvaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has been a traditional method for detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting of autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.

      A well established alternative to [3H] thymidine uptake has been demonstrated by numerous researchers. In these methods bromodeoxyuridine (BrdU), a thymidine analog, replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells which are synthesizing DNA.
      Materials Required but Not Delivered

      1. Pipettors & tips capable of accurately measuring 2-1000 μL

      2. Multi-channel or repeating pipettes

      3. Wash bottle or multichannel dispensor for washing

      4. 2000 mL graduated cylinder

      5. PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4)

      6. Deionized or distilled H2O.

      7. 96-well microplate reader with 450-540 nm or 450-595 nm dual wavelength or 450 nm single wavelength filter

      8. Tissue culture microplate (96 well culture dish)

      9. Sterile reagent troughs

      10. Micro syringe filter (0.2 mm)

      11. Syringe
      References
      Product Information
      Components
      • BrdU Reagent: (Part No. 2752a) One 100 μL vial of a 500X solution of BrdU.
      • Fixing Solution: (Part No. 2752b) One 200 mL bottle.
      • Prediluted BrdU Detection Antibody: (Part No. 2752c) One 100 mL vial of anti-BrdU in solution.
      • Stop Solution: (Part No. 2752d): One 100 mL bottle of 2.5N sulfuric acid solution.
      • Goat anti-Mouse IgG, Peroxidase labeled: (Part No. 2752e) One 60 μL vial of a 2000X solution.
      • Conjugate Diluent: (Part No. 2752f) One 100 mL bottle.
      • Substrate: (Part No. 2752g) One 100 mL bottle, ready to use TMB.
      • 50X Plate Wash Concentrate: (Part No. 2752h) One 125 mL bottle of buffered Tris with surfactant.
      Detection methodColorimetric
      Applications
      ApplicationThe BrdU Cell Proliferation Assay Kit is a non-isotopic assay for the in vitro quantitative detection of newly synthesized DNA of actively proliferating cells.
      Key Applications
      • ELISA
      Biological Information
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Pig
      • Horse
      • Rabbit
      • Guinea Pig
      • Hamster
      • Non-human Primate
      • Canine
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the unopened kit at 2-8°C until the expiration date indicated on the label.

      Before first use: remove the Fixative Solution and place at room temperature for at least 4 hours prior to use. Precipitates that may occur during cold storage should go back into solution.
      Packaging Information
      Material Size1000 assays
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      BrdU Cell Proliferation Kit MSDS

      Title

      Safety Data Sheet (SDS) 

      References

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