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3323 | LIGHT DIAGNOSTICS™ Enterovirus 71 / Coxsackie A16 Reagent, ~25 tests, included in kit #3345

3323
1 mL  
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      Key ApplicationsFormatHostDetection Methods
      IF Purified M Fluorescent
      Description
      Catalogue Number3323
      Brand Family Chemicon®
      Trade Name
      • LIGHT DIAGNOSTICS
      • Chemicon
      DescriptionLIGHT DIAGNOSTICS™ Enterovirus 71 / Coxsackie A16 Reagent, ~25 tests, included in kit #3345
      OverviewLight Diagnostics Enterovirus 71 Monoclonal Antibody is a type-specific reagent intended for use in indirect immunofluorescence screening for the presumptive identification of enterovirus 71 obtained in cell culture and not intended for testing directly on human specimens.

      For in vitro diagnostic use.

      Test Principle:

      Light Diagnostics Enterovirus 71 Monoclonal Antibody (MAb EV 71) can be used to identify an enterovirus 71 isolate in cell culture using an indirect immunofluorescence assay (IFA). The monoclonal antibody provided will bind to the type-specific enterovirus 71 isolate present in the cell culture slide. Unbound monoclonal antibody is removed by rinsing with phosphate buffered saline (PBS). A secondary FITC (fluorescein isothiocyanate) labeled antibody is then added which will bind to the antigen-antibody complex. Unbound secondary antibody is removed by rinsing with PBS. FITC exhibits an apple-green fluorescence when illuminated by ultraviolet light allowing visualization of the complex by microscopy. A positive result is indicated by specific cell fluorescence. Uninfected cells stain a dull red if Evans blue counterstain is used in the FITC-labeled secondary antibody or elsewhere in the procedure.

      Background and Clinical Significance:

      Enteroviruses, such as enterovirus 71, are classified to be in the picornavirus family, pico [small] + RNA [ribonucleic acid] + virus. Picornaviruses are among the smallest and simplest ribonucleic acid containing viruses known (1). The RNA for many enteroviruses have now been cloned and complete genomic sequences have been obtained. The RNA from all sequenced enteroviruses are similar in length, about 7400 nucleotides, and have identical organization (1).

      The human alimentary tract is the predominant site of enterovirus replication and these viruses were first isolated from enteric specimens. These viruses are the cause of paralytic poliomyelitis, aseptic meningitis-encephalitis, myocarditis, pleurodynia, hand-foot-and-mouth disease, conjunctivitis, and numerous other syndromes associated with extra-intestinal target organs. There are 67 numbered types of enteroviruses in the enterovirus family (1): three polioviruses, twenty-three coxsackieviruses A, six coxsackieviruses B, thirty-one echoviruses, and four other enteroviruses.

      Enteroviruses, including echoviruses and coxsackieviruses, have been reported as the major etiologic agents of aseptic meningitis (2). Clinical syndromes associated with infections by each type of enterovirus have also been reported (3). Enterovirus 71 can cause paralysis, meningoencephalitis, and hand-foot-and-mouth disease, as can the closely related coxsackievirus A16 (25).

      Establishing an association between an enterovirus and a particular disease in a patient requires laboratory confirmation of infection, usually by either isolation of the virus or documentation of a specific serologic response in a properly timed specimen. Detailed descriptions of principles and procedures for diagnosis of enterovirus infections have been published (4-7). Cell culture techniques have made the accurate detection of enteroviruses possible (8-10).

      The identification of the enterovirus isolates help in the prevention, treatment and understanding of the infectious diseases, and even discovery of new virus isolates. The typing of enterovirus isolates is generally accomplished by neutralization with type-specific pools of immune sera (11). This method is time consuming (7 days or more) and expensive. As an alternative, typing of enteroviruses with type-specific monoclonal antibody and/or group-specific monoclonal antibody pool(s) by indirect immunofluorescence is potentially more rapid and less expensive (12-18).
      References
      Product Information
      Components
      • Enterovirus 71 Monoclonal Antibody - (Catalog No. 3323)
      • One 1 mL dropper vial containing mouse IgG1 monoclonal antibody against enterovirus 71, protein stabilizer and 0.1 % sodium azide (preservative).
      Detection methodFluorescent
      FormatPurified
      PresentationMaterials Required But Not Provided:
      · Acetone, reagent grade; stored in glass

      · Distilled water or deionized water

      · Sodium hypochlorite solution, 0.05% (1:100 dilution of household bleach)

      · Sterile shell vials with 12 mm coverslips containing monolayer of cell line appropriate for growth of enteroviruses

      · Tissue culture media such as RPMI or Eagle's Minimum Essential Medium (EMEM) with fetal bovine serum (FBS) and antibiotics, or equivalent

      · Viral transport medium which is non-inhibitory to enteroviruses

      · 0.1N NaOH

      · 0.1N HCl

      · Microscope slides, non-fluorescing

      · No. 1 cover slips

      · Negative and positive control slides

      · Mounting Fluid (Catalog No. 5013)

      · Enterovirus 70/71 Control Slides (Catalog No. 5066)

      · Anti-Mouse IgG:FITC Conjugate (Catalog No. 5008)

      · Normal Mouse Antibody such as (Catalog No. 5014) as negative control

      · Phosphate buffered saline (PBS, 0.01 M pH 7.1 to 7.4 with 0.085% NaCl and 0.1% sodium azide), (Catalog No. 5087)

      · 0.05% Tween 20/0.1% Sodium Azide Solution (optional), (Catalog No. 5037)

      · Aspirator device with disposable sterile Pasteur pipettes

      · Centrifuge capable of 700 to 950 x g with biohazard buckets and adapters for shell vials

      · Fluorescence microscope with appropriate filter combination for FITC (excitation peak = 490 nm, emission peak = 520 nm) with 100x, 200x, 400x magnification (dry objective)

      · Forceps

      · Humid chamber

      · Incubator, 37 * 1*C

      · Syringe filter, 0.45 micron

      · Ultrasonic waterbath

      · Vortex mixer or sonicator
      Applications
      Key Applications
      • Immunofluorescence
      Biological Information
      HostMouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For in vitro Diagnostic Use
      • CE Mark
      Storage and Shipping Information
      Storage ConditionsWhen stored at 2-8°C, the monoclonal antibody is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures. discard any remaining reagent after the expiration date.

      Warning and Precautions:

      * The performance of Light Diagnostics Enterovirus 71 MAb has not been determined on direct specimens.

      · Sodium azide, present in the reagent, may react with lead and copper plumbing to form potentially explosive metal azides. When disposing of solutions that contain sodium azide, flush plumbing with a large volume of water to prevent build-up.

      · Handle all specimens and materials coming in contact with them as potentially infectious materials. Decontaminate with 0.05% sodium hypochlorite prior to disposal.

      · Avoid contact with Evans blue, if present in any reagent, as it is a potential carcinogen. If skin contact occurs, flush with large volumes of water.

      · Do not mouth pipette reagents.

      · Do not allow shell vials or slides to dry at any time during the staining procedure.

      · Pooling or alteration of any reagent may cause erroneous results.

      · Acetone is extremely flammable and harmful if swallowed or inhaled. Keep away from heat, sparks or flame. Avoid breathing vapor. Use adequate ventilation.

      · Mounting Fluid (Catalog No. 5013) contains a fluorescence enhancer that may be destructive to mucous membranes. Avoid direct skin or mucous membrane contact. If contact occurs, flush with large volumes of water.

      · Slides prepared too early (<25% CPE) or too late (>95% CPE) can be difficult to read and can lead to false negatives.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Acute chemokine response in the blood and cerebrospinal fluid of children with enterovirus 71-associated brainstem encephalitis.
      Shih-Min Wang, Huan-Yao Lei, Chun-Keung Yu, Jen-Ren Wang, Ih-Jen Su, Ching-Chuan Liu
      The Journal of infectious diseases 198 1002-6 2008

      Pokaż streszczenie
      18710325 18710325
      Critical management in patients with severe enterovirus 71 infection.
      Jieh-Neng Wang, Chih-Ta Yao, Cheng-Nan Yeh, Chao-Ching Huang, Shih-Min Wang, Ching-Chuan Liu, Jing-Ming Wu
      Pediatrics international : official journal of the Japan Pediatric Society 48 250-6 2006

      Pokaż streszczenie
      16732789 16732789
      Modulation of cytokine production by intravenous immunoglobulin in patients with enterovirus 71-associated brainstem encephalitis.
      Shih-Min Wang, Huan-Yao Lei, Mei-Chih Huang, Ling-Yao Su, Hui-Chen Lin, Chun-Keung Yu, Jung-Lung Wang, Ching-Chuan Liu
      Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 37 47-52 2006

      Pokaż streszczenie
      16861032 16861032
      Cardiac complications of enterovirus rhombencephalitis.
      Y C Fu, C S Chi, Y T Chiu, S L Hsu, B Hwang, S L Jan, P Y Chen, F L Huang, Y Chang
      Archives of disease in childhood 89 368-73 2004

      Pokaż streszczenie Pełny tekst artykułu
      15033850 15033850
      Acute flaccid paralysis in infants and young children with enterovirus 71 infection: MR imaging findings and clinical correlates.
      C Y Chen, Y C Chang, C C Huang, C C Lui, K W Lee, S C Huang
      AJNR. American journal of neuroradiology 22 200-5 2001

      Pokaż streszczenie
      11158910 11158910
      Comparison of heart failure in children with enterovirus 71 rhombencephalitis and cats with norepinephrine cardiotoxicity.
      Y-C Fu,C-S Chi,N-N Lin,C-C Cheng,S-L Jan,B Hwang,S-L Hsu,C-L Gong,Y-T Chen,Y-T Chiu
      Pediatric cardiology 27 2001

      Pokaż streszczenie
      16933070 16933070
      Neurologic complications in children with enterovirus 71 infection.
      C C Huang, C C Liu, Y C Chang, C Y Chen, S T Wang, T F Yeh
      The New England journal of medicine 341 936-42 1999

      Pokaż streszczenie
      10498488 10498488

      User Guides

      Title
      LIGHT DIAGNOSTICS™ ENTEROVIRUS 71 MONOCLONAL ANTIBODY multi languages
      LIGHT DIAGNOSTICS™ ENTEROVIRUS 71 MONOCLONAL ANTIBODY