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109261 Lugol's solution (diluted iodine-potassium iodide solution)

109261
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092611000 Glass bottle 1 l
1092612500 Glass bottle 2.5 l
Description
Catalogue Number109261
SynonymsIodine-potassium iodide solution
DescriptionLUGOL'S SOLUTION (DILUTED IODINE-POTASS
OverviewIn bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution - an aniline dye - in the first step. After the treatment with Gram's iod solution (Lugol’s solution), a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear orange. A 500 mL PE-bottle of Gram's Lugol's solution is sufficient for staining up to 250 slides. Lugol’s solution is also a part of the Gram-color stain set. It is an IVD & CE product.
References
Product Information
HS Code3822 00 00
Applications
ApplicationUsed to form a crystal violet dye-iodine complex in Gram staining
Biological Information
Physicochemical Information
Boiling point100 °C (1013 hPa)
Density1.01 g/cm3 (20 °C)
pH value3.5 (H₂O, 20 °C)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Storage class10 - 13 Other liquids and solids
WGKWGK 1 slightly hazardous to water
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red

Documentation

Lugol's solution (diluted iodine-potassium iodide solution) SDS

Title

Safety Data Sheet (SDS) 

Lugol's solution (diluted iodine-potassium iodide solution) Certificates of Analysis

Product Number PackagingSpecificationLot Number
1092611000 Glass bottlePDF Specification Document
1092612500 Glass bottlePDF Specification Document

Brochure

Title
Färben von Mikroorganismen
Products for Microscopy-The Color of Life Science

Instructions for Use

Title
109261 Lugol's solution (diluted iodine-potassium iodide solution)

Related Products & Applications

Alternative Products

Catalogue Number Description
100567 Lugol's solution

Related Products

Catalogue Number Description
100567 Lugol's solution
111885 Gram-color

Accessories (Primary)

Catalogue Number Description
109217 Gram's safranine solution
109218 Gram's crystal violet solution
110218 Gram's decolorizing solution
111885 Gram-color

Accessories (Secondary)

Catalogue Number Description
109217 Gram's safranine solution
109218 Gram's crystal violet solution

Related Products By: Application Facete

Categories

IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining