ECM550 QCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric

ECM550
1 plate  12 wells
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      Overview

      Replacement Information

      Key Spec Table

      Detection Methods
      Chromogenic
      Description
      Catalogue NumberECM550
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM ECMatrix Cell Invasion Assay, 24-well (8 µm), colorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade.

      The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrix™, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor. We examined the kit's performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3).

      The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.

      The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.

      Test Principle:

      The CHEMICON Cell Invasion Assay is performed in an Invasion Chamber, a 24-well tissue culture plate with 12 cell culture inserts. The inserts contain an 8 μm pore size polycarbonate membrane, over which a thin layer of ECMatrixTM is dried. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane.

      Application:

      The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell Invasion Assay Kit contains sufficient reagents for the evaluation of 12 samples.

      The CHEMICON Cell Invasion Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
      Materials Required but Not Delivered1. Serum Free Media

      2. Multichannel pipette and tips

      3. Fetal Bovine serum

      4. Tissue culture incubator

      5. Microscope or Standard microplate reader

      6. 10% Acetic acid

      7. Beaker of Water

      8. 96-well or other microtiter plate
      References
      Product Information
      Components
      • Invasion Chamber: (Part No. 70019) One 24-well plate with 12 ECMatrixTM layered cell culture inserts.
      • Cell Stain: (Part No. 20294) One 10 mL bottle.
      • Cotton Swabs: (Part No. 10202) 24 each.
      • Forceps: (Part No. 10203) One each.
      Detection methodChromogenic
      Applications
      ApplicationThe CHEMICON Cell Invasion Assay Kit uses a 24-well plate, with 8 um pores, which provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
      Application NotesDetection of migrating cells is via crystal violet stain.
      Biological Information
      Species Reactivity
      • Vertebrates
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore kit materials at 2-8°C for up to 6 months. Do not freeze.
      Packaging Information
      Material Size1 plate
      Material Package12 wells
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      BCAR1 protein plays important roles in carcinogenesis and predicts poor prognosis in non-small-cell lung cancer.
      Wei Huang,Bo Deng,Ru-Wen Wang,Qun-You Tan,Yong He,Yao-Guang Jiang,Jing-Hai Zhou
      PloS one  7  2012

      Show Abstract
      22558353 22558353
      Epigenetic disruption of cadherin-11 in human cancer metastasis.
      F Javier Carmona,Alberto Villanueva,August Vidal,Clara Mu,Sara Puertas,Rosa M Penin,Montserrat Gom,Amaia Lujambio,Josep M Piulats,Ricard Mes,Montse S,Manel Man,Enric Condom,Suzanne A Eccles,Manel Esteller,Clara Muñoz,Montserrat Gomà,Ricard Mesía,Montse Sánchez-Céspedes,Manel Manós
      The Journal of pathology  228  2012

      Show Abstract
      22374749 22374749
      The effect of a novel frizzled 8-related antiproliferative factor on in vitro carcinoma and melanoma cell proliferation and invasion.
      Kristopher R Koch,Chen-Ou Zhang,Piotr Kaczmarek,Joseph Barchi,Li Guo,Hanief M Shahjee,Susan Keay
      Investigational new drugs  30  2012

      Show Abstract
      21931970 21931970
      Combination of paclitaxel- and retinoic acid-incorporated nanoparticles for the treatment of cT-26 colon carcinoma.
      Hong GY, Jeong YI, Lee SJ, Lee E, Oh JS, Lee HC
      Archives of pharmacal research  34  407-17. Epub 2011 May 6.  2011

      Show Abstract
      21547672 21547672
      RhoBTB2 (DBC2) functions as tumor suppressor via inhibiting proliferation, preventing colony formation and inducing apoptosis in breast cancer cells.
      Mao H, Zhang L, Yang Y, Sun J, Deng B, Feng J, Shao Q, Feng A, Song B, Qu X
      Gene  486  74-80. Epub 2011 Jul 23.  2011

      Show Abstract
      21801820 21801820
      Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway.
      Dan Liu,Yi Huang,Jing Zeng,Bojiang Chen,Na Huang,Na Guo,Lunxu Liu,Hong Xu,Xianming Mo,Weimin Li
      Journal of cancer research and clinical oncology  137  2011

      Show Abstract
      21861134 21861134
      Mullerian Inhibiting Substance inhibits invasion and migration of epithelial cancer cell lines.
      Chang HL, Pieretti-Vanmarcke R, Nicolaou F, Li X, Wei X, Maclaughlin DT, Donahoe PK
      Gynecol Oncol  2010

      Show Abstract
      21056908 21056908
      The inhibitory mechanism of a novel cationic glucosamine derivative against MMP-2 and MMP-9 expressions.
      Eresha Mendis, Moon-Moo Kim, Niranjan Rajapakse, Se-Kwon Kim
      Bioorganic medicinal chemistry letters  19  2755-9  2009

      Show Abstract
      19375915 19375915
      Antitumor effect of all-trans retinoic acid-encapsulated nanoparticles of methoxy poly(ethylene glycol)-conjugated chitosan against CT-26 colon carcinoma in vitro.
      Jung-Sun Park, Yang-Seok Koh, Je-Yong Bang, Young-Il Jeong, Je-Jung Lee
      Journal of pharmaceutical sciences  97  4011-9  2008

      Show Abstract
      18240304 18240304
      Pancreatic stellate cells promote proliferation and invasiveness of human pancreatic cancer cells via galectin-3.
      Hai-Biao Jiang,Ming Xu,Xing-Peng Wang
      World journal of gastroenterology : WJG  14  2008

      Show Abstract Full Text Article
      18395901 18395901

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression
      Cell Migration and Invasion: Choosing the Right Assay

      Data Sheet

      Title
      QCM ECMatrix Cell Invasion Assay, Colorimetric - Data Sheet
      QCM ECMatrix Cell Invasion Assay, Colorimetric - Data Sheet
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      Posters

      Title
      Poster: Tumor Metastasis

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      Categories

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