ECM580 QCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric

ECM580
1 kit  Sufficient for analysis of 12 samples
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      Overview

      Replacement Information

      Key Spec Table

      Detection Methods
      Chromogenic
      Description
      Catalogue NumberECM580
      ReplacesECM500
      Brand Family Chemicon®
      Trade Name
      • QCM
      • Chemicon
      DescriptionQCM Haptotaxis Cell Migration Assay -Fibronectin, 24-well, colorimetric
      OverviewAlso available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay! Click Here

      Introduction
      The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric is ideal for the study of epithelial and fibroblast cell haptotaxis. The quantitative nature of this assay is especially useful for large scale screening of pharmacologic agents. BSA-coated control chambers provide an appropriate migration control. The 8 μm pore size of this assay's Boyden chambers is not appropriate for the study of lymphocyte migration.
      • Each kit provides sufficient materials for the evaluation of 12 samples.
      • Please refer to the product insert for assay details.

      The CHEMICON QCM™ Haptotaxis Cell Migration Assay - Fibronectin, Colorimetric assay is intended for research use only; not for diagnostic applications.
      Materials Required but Not Delivered1. Precision pipettes: sufficient for aliquoting cells.
      2. Harvesting buffer: EDTA or trypsin based cell detachment buffer, or other cell detachment formulations as optimized by individual investigators. Chemicon's ready-to-use non-mammalian detachment solution, Accutase™ (Cat. No. SCR005) can also be used.
      3. Tissue culture growth medium appropriate for subject cells, such as DMEM containing 5% FBS.
      4. Quenching Medium: serum-free medium, such as DMEM, MEM etc containing 5% BSA. Must contain divalent cations (Mg2+, Ca2+) sufficient for quenching EDTA in harvesting buffer.
      5. Sterile PBS or HBSS to wash cells.
      6. Distilled water
      7. (Optional) Chemoattractant or pharmacological agent added to culture medium.
      8. Low speed centrifuge and tubes for cell harvesting.
      9. CO2 incubator appropriate for subject cells.
      10. Hemocytometer or other means of counting cells.
      11. Trypan blue or equivalent viability stain.
      12. Microplate reader (540-570 nm detection) or spectrophotometer.
      13. Sterile cell culture hood
      14. (Optional) Graduated ocular (calibrated), or automated method for counting stained cells on a membrane.
      15. Shaker
      References
      Product Information
      Components
      • Fibronectin Test Plate: (Part No. 2005689) One 24-well culture plate, containing 12 human FN-coated Boyden chambers, sufficient for the evaluation of 12 test samples.
      • BSA Control Plate: (Part No. 2005791) One 24-well culture plate, containing 12 BSA-coated Boyden chambers, sufficient for the evaluation of 12 controls.
      • Cell Stain Solution*: (Part No. 20294) One vial - 10 mL.
      • Extraction Buffer: (Part No. 20295) One vial - 10 mL.
      • 24 well Stain Extraction Plate: (Part No. 2005871). One plate.
      • 96 well Stain Quantitation Plate: (Part No. 2005870). One plate.
      • Swabs: (Part No. 10202) 50 each.
      • Forceps: (Part No. 10203) 1 pair
      • *Cell Stain Solution contains a small amount of crystal violet, which is toxic if swallowed or inhaled, and may cause irritation to the eyes, respiratory system, and skin. Handle with caution.
      Detection methodChromogenic
      Applications
      ApplicationThe QCM Haptotaxis Cell Migration Assay, 24-well plate -Fibronectin, Colorimetric is ideal for the study of epithelial & fibroblast cell haptotaxis.
      Biological Information
      Species Reactivity
      • Vertebrates
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 4°C for up to one year after date of purchase.
      Packaging Information
      Material Size1 kit
      Material PackageSufficient for analysis of 12 samples
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays