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03-0047-00 SMC™ Human Granulocyte Colony-Stimulating Factor (G-CSF)
IMMUNOASSAY KIT

03-0047-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey Applications
G-CSFHSingle Molecule Counting (SMC™)
Description
Catalogue Number03-0047-00
Trade Name
  • SMC™
DescriptionSMC™ Human Granulocyte Colony-Stimulating Factor (G-CSF)
IMMUNOASSAY KIT
OverviewThe SMC™ G-CSF Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationGranulocyte-Colony Stimulating Factor (G-CSF) is an endogenous hematopoietic agent activated and expressed by many types of cells, including macrophages, tumor cells, fibroblasts, and endothelial cells. One of the main functions of G-CSF is to regulate formation of neutrophils during hematopoiesis, which are important for proper immune function. For example, in cancer patients reduced hematopoeisis from chemotherapy results in low neutrophil production, which jeopardizes the immune system, leaving the body susceptible to infectious attacks. G-CSF therapy has been shown to restore hematopoiesis in cancer and leukemic patients by stimulating white blood cell production. Interestingly, G-CSF has also demonstrated effectiveness in improving cardiac function following a myocardial infarction.
References
Product Information
Components
  • G-CSF Coated Beads
  • Standard Diluent
  • G-CSF Detection Antibody
  • Assay Buffer
  • Human G-CSF Standard
  • SMC™ Human G-CSF Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer D
Precision, %
  • Low-end CV% Range: 5 - 9%
  • Low-end CV% Average: 7%
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ G-CSF Immunoassay Kit unparalleled performance in the quantification of G-CSF in human EDTA plasma.
Key Applications
  • Single Molecule Counting (SMC™)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • G-CSF
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Erenna SMC¿ Human G-CSF Immunoassay Kit Instructions

SMC™ Human Granulocyte Colony-Stimulating Factor (G-CSF)
IMMUNOASSAY KIT SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Singulex Erenna® Immunoassay System

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods