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03-0049-00 SMC™ Human Interferon-gamma (IFN-γ)
IMMUNOASSAY KIT

03-0049-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey Applications
IFN-γHSingle Molecule Counting (SMC™)
Description
Catalogue Number03-0049-00
Trade Name
  • SMC™
DescriptionSMC™ Human Interferon-gamma (IFN-γ)
IMMUNOASSAY KIT
OverviewThe SMC™ Human IFN-γ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterferon-gamma (IFN-γ) is a cytokine with anti-viral and tumor-suppressing properties that is actively involved in the innate immune system. As the only member of the Type 2 IFN family, IFN-γ is expressed by Th1 cells, Tc cells, dendritic cells, and natural killer cells. Once bound to its heterodimeric receptor IFN-γR, the activated glycoprotein recruits and directs macrophages to the site of infection and escalates the inflammatory response. IFN-γ has also been documented to up-regulate the expression of IL-1 and down-regulate the production of IL-8, both of which are pro-inflammatory cytokines. The potency, specificity and rapid action of IFN-γ make it an attractive drug target for immuno-regulatory diseases.
References
Product Information
Components
  • IFN-γ Coated Beads
  • Standard Diluent
  • IFN-γ Detection Antibody
  • Assay Buffer
  • Human IFN-γ Standard
  • SMC™ Human IFN-γ Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer D
Precision, %
  • Low-end CV% Range: 1 - 12%
  • Low-end CV% Average: 8%
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IFN-γ Immunoassay Kit unparalleled performance in the quantification of IFN-γ in human EDTA plasma.
Key Applications
  • Single Molecule Counting (SMC™)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • IFN-γ
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Erenna SMC¿ Human IFN-γ Immunoassay Kit Instructions

SMC™ Human Interferon-gamma (IFN-γ)
IMMUNOASSAY KIT SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Singulex Erenna® Immunoassay System

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods