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03-0025-06 SMC™ Human Total Glucagon-Like Peptide-1 (Total GLP-1)
IMMUNOASSAY KIT

03-0025-06
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey Applications
GLP-1 (Total)H, M, R, CaSingle Molecule Counting (SMC™)
Description
Catalogue Number03-0025-06
Trade Name
  • SMC™
DescriptionSMC™ Human Total Glucagon-Like Peptide-1 (Total GLP-1)
IMMUNOASSAY KIT
OverviewThe SMC™ Total GLP-1 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationGlucagon-Like Peptide-1 (GLP-1) is a member of the incretin family of hormones, which are released by the gut into the bloodstream in response to food. Post-translational cleavage of the peptide produces active GLP-1, which is then rapidly degraded by protease dipeptidyl peptidase-4 (DPP-4). When blood glucose level is high, active GLP-1 enhances insulin secretion and also suppresses the release of glucagon. In addition, GLP-1 stimulates the brain to trigger feelings of satiety and regulates the rate of gastric emptying. Compared to healthy controls, some patients with type-2 diabetes show a modest decrease in GLP-1 release. Therapeutic GLP-1 analogs such as exenatide, or DPP inhibitors like sitagliptin, are promising strategies for treating type-2 diabetes.
References
Product Information
Components
  • Total GLP-1 Coated Beads Vial 1
  • Total GLP-1 Coated Beads Vial 2
  • Total GLP-1 Standard Diluent
  • GLP-1 Assay Buffer
  • Total GLP-1 Standard
  • Total GLP-1 Detection Antibody
  • SMC™ Total GLP-1 Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer D
Precision, %
  • Low-end CV% Range: 5 - 12%
  • Low-end CV% Average: 9%
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the Total GLP-1 Immunoassay Kit unparalleled performance in the quantification of total GLP-1 in human EDTA plasma.
Key Applications
  • Single Molecule Counting (SMC™)
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Canine
Analytes Available
  • GLP-1 (Total)
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Erenna SMC¿ Total GLP-1 Immunoassay Kit Instructions

SMC™ Human Total Glucagon-Like Peptide-1 (Total GLP-1)
IMMUNOASSAY KIT SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Singulex Erenna® Immunoassay System

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods