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03-0068-00 SMC™ Human Vascular Endothelial Growth Factor (VEGF)
IMMUNOASSAY KIT

03-0068-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey Applications
HSingle Molecule Counting (SMC™)
Description
Catalogue Number03-0068-00
Trade Name
  • SMC™
DescriptionSMC™ Human Vascular Endothelial Growth Factor (VEGF)
IMMUNOASSAY KIT
OverviewThe SMC™ Human VEGF Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationVEGF-A, commonly known as VEGF, is one member of a family of secreted glycoproteins that promote endothelial cell growth, survival, migration, and vascular permeability, all of which contribute to angiogenesis. The binding of VEGF to its receptor triggers the activation of a cell signaling pathway that is critical for the growth of blood vessels from pre-existing vasculature. VEGF is implicated in a variety of diseases, including several cancers, age-related macular degeneration, diabetic retinopathy and rheumatoid arthritis. With the efficacy seen with drugs that target VEGF and its pathway, VEGF will remain an attractive candidate for the development of therapies for these diseases, particularly cancer.
References
Product Information
Components
  • VEGF Coated Beads
  • Standard Diluent
  • VEGF Detection Antibody
  • Assay Buffer
  • VEGF Standard
  • SMC™ Human VEGF Immunoassay Kit Instructions
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer D
Precision, %
  • Low-end CV% Range: 2 - 15%
  • Low-end CV% Average: 7%
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human VEGF Immunoassay Kit unparalleled performance in the quantification of VEGF in human EDTA plasma.
Key Applications
  • Single Molecule Counting (SMC™)
Biological Information
Species Reactivity
  • Human
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Erenna SMC¿ Human VEGF Immunoassay Kit Instructions

SMC™ Human Vascular Endothelial Growth Factor (VEGF)
IMMUNOASSAY KIT SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
Singulex Erenna® Immunoassay System

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods

Related Products & Applications

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Categories

Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits