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MILLIPLEX® Multiplex Assays Using Luminex® |
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As your research uncovers the significant role of more and more biomarkers in both normal and disease states, you need a reliable partner for quality assays and consistent performance. Our MILLIPLEX® MAP portfolio of immunoassays is the largest portfolio of multiplex biomarker assays, based on Luminex® xMAP® technology, offering you consistent, high-quality results, so that you can do your best work while saving time, labor and cost.
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Featured Customer Story
Interested in how MILLIPLEX® MAP assays can further Zika research?The MILLIPLEX® MAP Rat Cytokine Panel (Cat. No. RECYTMAG-65K) allowed Dr. Mukesh Kumar, of the University of Hawaii, to develop a guinea pig model for Zika virus infection. Learn more about his research by viewing the video below, and read his most recent publication.
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Need Tips on Optimizing your Multiplex Assays?
Read the SelectScience Interview with Sonali Nayak, Bioscience Technical Services Manager, Merck:Discover How Multiplex Assays are Advancing Biomedical Research: Top tips to optimize your analyses
Get Sonali Nayak’s take on how to best run your MILLIPLEX® MAP assays.
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MILLIPLEX® MAP Kit Validation and Quality Assurance Criteria
We carefully develop and validate every MILLIPLEX® MAP kit and evaluate performance criteria including cross-reactivity, sensitivity, specificity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). We write the protocol with assay performance and your workflow in mind, making it easy to find the information you need.Each MILLIPLEX® MAP kit includes all necessary reagents to prepare the assay to read on your Luminex® instrument, just add your sample and water:
- Selectable capture antibody-conjugated magnetic beads in solution
- A premixed standard cocktail allowing you to easily rehydrate and prepare your serial dilutions
- Two premixed quality control cocktails (QCs) to qualify assay performance
- A premixed detection antibody cocktail designed to yield consistent analyte profiles within panel
- A prediluted optimized concentration of Streptavidin Phycoerythrin (SAPE)
- An optimized serum matrix is included for kits validated for serum or plasma samples, to mimic native sample environment
- Bead diluent containing proprietary blocking reagents to reduce non-specific binding to the antibodies in the assay
- 10X wash buffer and assay buffer optimized specifically for the kit
- 96- or 384-well plate (depending on assay format) with two plate sealers
MILLIPLEX® MAP cell signaling kits have a different format to support the use of cell lysate samples.
If you require analytes that are not in our portfolio, or different combinations of analytes, please visit our Custom Immunoassay Development Services page.
How to Decide Which MILLIPLEX® Assay Is Best for Your Research
In Method Comparison tests (comparing ELISA and MILLIPLEX® MAP assays), while the absolute values are not exactly the same, the results do correlate. Hence when switching from one assay method to another, a correction factor may be used when comparing with past data. In most situations the dynamic range and the sensitivity of the assay may be better with the MILLIPLEX® kits.Questions? Visit our MILLPLEX® Frequently Asked Questions page. If you have a specific question, or one we did not answer, please contact our Technical Support Team directly.
Request the Analyte Quarterly brochure for a complete, updated list of assays.
Multiplex Customer Testimonial
What are researchers saying about MILLIPLEX® MAP Quality?
“I have used Merck MILLIPLEX® MAP kits for the past four years. Initially I started using these kits to multiplex a specific set of markers, but given their reliability, consistency, and ease of use, I have transitioned all my experiments away from traditional ELISAs. Beyond just being a great product, Merck's customer and technical support is at the top of the industry. The combination of superior product with award-winning support has extended the capabilities of my research, allowing me to answer new novel research questions. I consider MILLIPLEX® MAP kits an important component of my research toolbox.”Brian McFarlin, PhD, FACSM, University of North Texas, Denton, Texas
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