MAB5326B Anti-Nestin Antibody, clone 10C2, Biotin Conjugate

MAB5326B
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H ICC, IHC M Biotin Monoclonal Antibody
      Description
      Catalogue NumberMAB5326B
      DescriptionAnti-Nestin Antibody, clone 10C2, Biotin Conjugate
      Alternate Names
      • Nestin10c2
      • NES
      • Nbla00170
      • FLJ21841
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
      References
      Product Information
      FormatBiotin
      Control
      • ReNcell VM neural stem cells (SCC008)
      PresentationPurified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Applications
      ApplicationDetect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone 10C2, Biotin Conjugate validated for use in ICC & IHC.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesEvaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in rat hippocampal neural stem cells (SCR022). Evaluated by Immunohistochemistry in human gliomablastoma tissue. Immunohistochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human gliomablastoma tissue.
      Biological Information
      ImmunogenFusion protein corresponding to human Nestin.
      Clone10C2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes human Nestin. Antibody is not reactive with rat or mouse Nestin protein.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NES
      • Nbla00170
      • FLJ21841
      • Nestin.
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight200-220 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in Human ReNcell VM neural stem cells (SCC008). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in Human ReNcell VM neural stem cells (SCC008).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications