CF200097 Autophagy Detection Reagent Pack

CF200097
100 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCF200097
      DescriptionAutophagy Detection Reagent Pack
      OverviewMillipore’s Autophagy Detection Reagent Pack provides all of the required reagents and buffers to perform the selective permeabilization technique for the study of autophagy by flow cytometry. This reagent pack is designed to be used with the current Autophagy reporter cell line kits listed as items 1-3 in the related products section below. The Autophagy Detection Reagent Pack extends the usage of the reporter cell lines in these kits, once the original reagent pack is finished. Thus it provides a very cost-effective format.
      Alternate Names
      • FlowCellect
      Background InformationAutophagy is an intracellular catabolic pathway which causes cellular protein and organelle turnover, and is associated with a diverse range of diseases including Alzheimer's disease, aging-related processes, cancers, and Crohn's disease. It is a tightly regulated process that plays a normal part in cell growth, development, and cellular homeostasis. Autophagy functions as a housekeeping mechanism through disposal of aging and/or dysfunctional proteins and organelles by sequestering and priming these proteins for lysosomal degradation. Increasing evidence suggests that autophagy, in addition to apoptosis, can contribute to cell death and greatly influence general cell health. Malfunctions of the autophagy process are hypothesized to influence cell health, cellular longevity and the ability of cells to function at full capacity.
      References
      Product Information
      Components
      • 1. Autophagy reagent A: (Part No. CS208212) One vial (lyophilized)
      • 2. Autophagy reagent B: (Part No. CS208215) One vial containing 1 mL
      • 3. 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL
      Detection methodFluorescent
      FormatCell Line
      Instrument compatibility
      • EasyCyte 8HT
      • EasyCyte
      Applications
      ApplicationThis Autophagy Detection Reagent Pack provides all of the required reagents & buffers to perform the selective permeabilization technique for the study of autophagy by flow cytometry.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAll reagents must be stored at 2 - 8ºC. All kit components are stable up to six (6) months from date of receipt if stored and handled correctly. Please avoid exposure of the product to repeated changes in temperature as this will affect its integrity.
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Accessories

      Catalogue Number Description  
      FCCH100170 FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (CHO) Show Pricing & Availability
      FCCH100171 FlowCellect™ Autophagy LC3 Antibody-based Assay Kit (100 tests) Show Pricing & Availability
      FCCH100183 FlowCellect™ RFP-LC3 Reporter Autophagy Assay Kit Show Pricing & Availability

      Software Compatibility

      Catalogue Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents