FCCH100110 FlowCellect Cytochrome C Kit

FCCH100110
100 Tests  
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      Overview

      Replacement Information
      Description
      Catalogue NumberFCCH100110
      Trade Name
      • FlowCellect
      DescriptionFlowCellect Cytochrome C Kit
      OverviewMillipore’s FlowCellect™ Cytochrome c Kit assesses the loss of mitochondrial Cytochrome c in cells that have been induced to undergo apoptosis. The kit includes a directly labeled Anti-Cytochrome c-FITC Antibody, Anti-IgG1-FITC Isotype control, along with optimized fixation, permeabilization, and blocking buffers to allow the detection of Cytochrome c using flow cytometry. The assay kit may be used on a flow cytometer equipped with a 488 nm laser which allows the detection of FITC probes. The kit uses specialized buffer sets to achieve selective permeabilization of mitochondira while leaving the mitochondrial membrane intact. Viable or live cells will demonstrate higher levels of Cytochrome c staining while apoptotic cells which have released their Cytochrome c from the mitochondria to the cytoplasm will demonstrate reduced staining intensity when probed with an anti-Cytochrome c FITC antibody. Stained cells can then be analyzed by flow cytometry. The entire staining can be performed in 2 hours once cells are ready. Sufficient reagents are provided for 100 samples. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for cell preparation and analysis. This flow cytometry method can be stained and analyzed within 3 hours of completion of induction for as many as 96 samples.
      Alternate Names
      • FlowCellect
      References
      Product Information
      Components
      • Anti-Cytochrome C -FITC
      • Anti-IgG1 - FITC
      • 10X Permeabilization Buffer
      • 5X Fixation Buffer
      • 1X Blocking Buffer
      • User Manual
      Detection methodFluorescent
      Instrument compatibility
      • EasyCyte 8HT
      • EasyCyte Plus
      • EasyCyte Mini
      • EasyCyte
      Applications
      ApplicationThis FlowCellect Cytochrome c Kit for flow cytometry assesses the loss of mitochondrial Cytochrome c in cells that have been induced to undergo apoptosis.
      Key Applications
      • Flow Cytometry
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Software Compatibility

      Catalogue Number Description  
      0500-4120 InCyte Software for Guava® easyCyte HT Systems Show Pricing & Availability

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents