48-680MAG MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay

48-680MAG
96-well plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (pT180/pY182), p70 S6K (pT412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473)HMplexLuminex xMAP
      Description
      Catalogue Number48-680MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® MAP 9-plex Multi-Pathway Signaling Magnetic Bead kit, phosphoprotein, is used to detect changes in phosphorylated ERK/MAP kinase 1/2 (Thr185/Tyr187), Akt (Ser473), STAT3 (Ser727), JNK (Thr183/Tyr185), p70 S6 kinase (Thr412), NFkB (Ser536), STAT5A/B (Tyr694/699), CREB (Ser133), and p38 (Thr180/Tyr182) in cell lysates using the Luminex® xMAP® technology. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.

      References:
      Pharmacol Res. 2016 Sep 12;113(Pt A):356-363. doi: 10.1016/j.phrs.2016.08.028. [Epub ahead of print]
      The endocannabinoid anandamide causes endothelium-dependent vasorelaxation in human mesenteric arteries.
      Stanley CP, Hind WH, Tufarelli C, O'Sullivan SE.

      PLoS One. 2015 Sep 28;10(9):e0136546. doi: 10.1371/journal.pone.0136546. eCollection 2015.
      The Effects of the Endocannabinoids Anandamide and 2-Arachidonoylglycerol on Human Osteoblast Proliferation and Differentiation.
      Smith M, Wilson R, O'Brien S, Tufarelli C, Anderson SI, O'Sullivan SE.

      Cardiovasc Res. 2015 Sep 1;107(4):568-78. doi: 10.1093/cvr/cvv179. Epub 2015 Jun 19.
      Cannabidiol causes endothelium-dependent vasorelaxation of human mesenteric arteries via CB1 activation.
      Stanley CP, Hind WH, Tufarelli C, O'Sullivan SE.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Panel TypeCell Signaling
      Applications
      ApplicationAnalyze a cell signaling panel using this kit. The analytes available for the cell signaling multiplex assay kit are: CREB (pS133), ERK (pT185/pY187), NFκB (pS536), JNK (pT183/pY185), p38 (pT180/pY182), p70 S6K (pT412), STAT3 (pS727), STAT5A/B (pY694/699), Akt (pS473).
      Key Applications
      • Multiplexing
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • CREB (pS133)
      • ERK (pT185/pY187)
      • NFκB (pS536)
      • JNK (pT183/pY185)
      • p38 (pT180/pY182)
      • p70 S6K (pT412)
      • STAT3 (pS727)
      • STAT5A/B (pY694/699)
      • Akt (pS473)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Multi-Pathway 9-plex Magnetic Bead Panel

      MILLIPLEX MAP Multi-Pathway Magnetic Bead 9-Plex - Cell Signaling Multiplex Assay SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Upregulation of SOCS-1 by Nutlin-3 in acute myeloid leukemia cells but not in primary normal cells.
      Tisato, V; Norcio, A; Celeghini, C; Milani, D; Gonelli, A; Secchiero, P
      Clinics (São Paulo, Brazil)  69  68-74  2014

      Show Abstract
      24473562 24473562
      EMP1, a novel poor prognostic factor in pediatric leukemia regulates prednisolone resistance, cell proliferation, migration and adhesion.
      Ariës, IM; Jerchel, IS; van den Dungen, RE; van den Berk, LC; Boer, JM; Horstmann, MA; Escherich, G; Pieters, R; den Boer, ML
      Leukemia  2014

      Show Abstract
      24625531 24625531
      Lipopolysaccharide-binding protein inhibits toll-like receptor 2 activation by lipoteichoic acid in human odontoblast-like cells.
      Carrouel, F; Staquet, MJ; Keller, JF; Baudouin, C; Msika, P; Bleicher, F; Alliot-Licht, B; Farges, JC
      Journal of Endodontics  39  1008-14  2013

      Show Abstract
      23880268 23880268
      Inhibitory effect of natural anti-inflammatory compounds on cytokines released by chronic venous disease patient-derived endothelial cells.
      Tisato, V; Zauli, G; Rimondi, E; Gianesini, S; Brunelli, L; Menegatti, E; Zamboni, P; Secchiero, P
      Mediators of inflammation  2013  423407  2013

      Show Abstract
      24489443 24489443

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      Custom Immunoassay Menu of Services
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX® MAP Cell Signaling and Cytokine Panels
      MILLIPLEX® MAP Cell Signaling Wall Chart (MM)