FCMAB149F Milli-Mark Anti-BMI1 -FITC Antibody, clone AF27

FCMAB149F
100 tests  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB149F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-BMI1 -FITC Antibody, clone AF27
      Alternate Names
      • B lymphoma Mo-MLV insertion region 1 homolog
        B lymphoma Mo-MLV insertion region 1 homolog (mouse)
        BMI1 polycomb ring finger oncogene
        Polycomb group RING finger protein 4
        RING finger protein 51
        murine leukemia viral (bmi-1) oncogene homolog
        oncogene BMI-1
        polycomb group protein Bmi1
        polycomb group ring finger 4
      Background InformationThe BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1.
      References
      Product Information
      FormatFITC
      Control
      • HeLa cells.
      PresentationPurified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Applications
      ApplicationMilli-Mark Anti-BMI1 -FITC Antibody, clone AF27 detects level of BMI1 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenMBP-tagged recombinant protein corresponding to full length human BMI1.
      CloneAF27
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibody recognizes BMI1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MGC12685
      • PCGF4
      • RNF51
      • flvi-2/bmi-1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

      SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm.

      PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.

      INVOLVEMENT IN DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.

      SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger
      Molecular Weight 35kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Milli-Mark Anti-BMI1 -FITC, clone AF27 - 19750841975084
      Milli-Mark Anti-BMI1 -FITC, clone AF27 - 19989421998942
      Milli-Mark Anti-BMI1 -FITC, clone AF27 - 21682802168280
      Milli-Mark Anti-BMI1 -FITC, clone AF27 - NG1925929NG1925929
      Milli-Mark Anti-BMI1 -FITC, clone AF27 - NG1952999NG1952999
      Milli-Mark Anti-BMI1 -FITC, clone AF27 - NRG1724884NRG1724884
      Milli-Mark™ Anti-BMI1 -FITC, -26032362603236
      Milli-Mark™ Anti-BMI1 -FITC, clone AF272482254

      Brochure

      Title
      Innovative Solutions for Flow Cytometry Analysis

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies