MIT1000 Mitochondria/Cytosol Fractionation Kit

MIT1000
1 kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMIT1000
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMitochondria/Cytosol Fractionation Kit
      OverviewMitochondria, sometimes described as the “power plants” of the cell, are sites where most of the energy production in eukaryotic cells takes place. The synthesis of most of the adenosine triphosphate (ATP) occurs in these double membraned organelles that are found in living cells. ATP production by the mitochondria is done by the process of respiration, which uses oxygen to generate energy. This is a very efficient process for using food energy to make ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria also regulate crucial apoptosis signaling pathways. The number of mitochondria in a cell varies widely by organism and tissue type. Many cells have only a few mitochondria, whereas others can contain several thousand.

      Millipore’s Mitochondria/Cytosol Fractionation Kit provides reagents for quick and efficient isolation of intact mitochondria from cultured cells. This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction). Such separation is useful for studying apoptosis and signaling pathways between the two fractions, by Western blotting or ELISA.

      The kit contains sufficient materials for the analysis of 50 samples.
      References
      Product Information
      Components
      • Isotonic Mitochondrial Buffer (CS204255): 50 mL
      • Mitochondrial Lysis Buffer (CS204257): 5 mL
      • Protease inhibitor cocktail (CS204253): 1 mL
      • Anti-Bcl2 (05-729-25UG): 25 μg
      • Anti-GAPDH (CS204254): 25 μg
      Applications
      ApplicationThis Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.
      Key Applications
      • Cell Culture
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAll components should be store at -20°C for up to one year from date of receipt.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Mitochondria/Cytosol Fractionation Kit MSDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Quercetin induces protective autophagy in gastric cancer cells: involvement of Akt-mTOR- and hypoxia-induced factor 1α-mediated signaling.
      Kui Wang,Rui Liu,Jingyi Li,Jiali Mao,Yunlong Lei,Jinhua Wu,Jun Zeng,Tao Zhang,Hong Wu,Lijuan Chen,Canhua Huang,Yuquan Wei
      Autophagy  7  2011

      Show Abstract
      21610320 21610320

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

      User Guides

      Title
      Mitochondria/Cytosol Fractionation Kit

      Related Products & Applications

      Product Families

      Categories

      Life Science Research > Protein Sample Preparation > Protein Extraction > Specialized Extraction Kits