69952 pET-31b(+) DNA - Novagen

69952
  
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      Overview

      Replacement Information
      Description
      OverviewThe pET-31b(+) vector is designed for cloning and high-level expression of peptide sequences fused with the 125aa ketosteroid isomerase protein (1). The unique AlwN I cloning site allows the unidirectional insertion of of tandemly repeated peptide coding regions separated by methionine codons. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB098).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69952
      Brand Family Novagen®
      References
      References1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.
      Product Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Supplemental Information
      Specifications

      Documentation

      pET-31b(+) DNA - Novagen MSDS

      Title

      Safety Data Sheet (SDS) 

      pET-31b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69952

      References

      Reference overview
      1. Kuliopulos, A. and Walsh, C.T. (1994) J. Am. Chem. Soc. 116, 4599–4607.

      Brochure

      Title
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      Citations

      Title
    • Graeme S. Garvey, Susan P. McCormick and Ivan Rayment. 2008. Journal of Biological Chemistry 283, 1660.
    • Kim, M-J., et al. 2007. Journal of Biological Chemistry 282, 14253.
    • Ames, J.B., et al. 2006. Journal of Biological Chemistry 281, 37237.
    • Beloqui, A., et al. 2006. Journal of Biological Chemistry 281, 22933.
    • Pimentel, C., et al. 2006. Protein Science 15, 628.
    • Baneres, J-L., et al. 2005. Journal of Biological Chemistry 280, 20253.
    • Thai, K., et al. 2005. Protein Science 14, 948.
    • Juan Carlos Rodriguez, Lilly Wong and Patricia A. Jennings. 2003. Protein Expression and Purification 28, 224.
    • User Protocols

      Title
      TB055 pET System Manual
      TB104 pET Peptide Expression System 31

      Vector Map

      Title
      TB098VM pET-31b(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors