03-0164-00 SMC™ Human IL-17F High Sensitivity Immunoassay Kit

03-0164-00
96 well assay  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey Applications
      NhPSingle Molecule Counting (SMC™)
      Description
      Catalogue Number03-0164-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human IL-17F High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human IL-17F High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationIL-17F is a cytokine expressed by activated T cells and stimulates the production of other cytokines, such as IL-6, IL-8 and GM-CSF. It has been shown to play a role in cartilage matrix turnover, T-cell proliferation and angiogenesis. It shares 50% sequence homology with IL-17A and induces expression of proinflammatory cytokine and chemokines by interacting with receptors IL-17RA and IL-17RC. Mutations of the IL-17F gene have been linked to asthma and chronic pulmonary obstructive disease. As well, IL-17F has been linked to rheumatoid arthritis and alcoholic fatty liver disease.
      References
      Product Information
      Components
      • Assay Buffer
      • Human IL-17F Coated Beads
      • Standard Diluent
      • Human IL-17F Detection Antibody
      • Human IL-17F Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • Intra-assay CV% average (n=20): <10%
      • Inter-assay CV% average (n=5): <15%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-17F High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-17F in human serum and plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-17F kit, #03-0102-00:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Removal of a plate transfer step
      • Overnight incubation can be used
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity
      • Non-human Primate
      Species Reactivity NoteAlthough IL-17F was detectable in non-human primate samples, the assay has not been validated for this species.
      Accuracy
      • Minimum of 5 serum samples spiked with average recovery of 94% with a range of 86-100%
      • Minimum of 5 plasma samples spiked with average recovery of 105% with a range of 92-117%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.2 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications