03-0056-00 SMC™ Human Interleukin 10 (IL-10)
IMMUNOASSAY KIT

03-0056-00
96 well assay  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-10HSingle Molecule Counting (SMC™)
      Description
      Catalogue Number03-0056-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Interleukin 10 (IL-10)
      IMMUNOASSAY KIT
      OverviewThe SMC™ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin-10 (IL-10) is a cytokine with both stimulatory and suppressive effects on the immune system. The protein originates from several cell lines, including Th2 cells, mast cells, macrophages, monocytes and B-cells. Its main functions are to impede inflammatory responses and to inhibit synthesis of other pro-inflammatory cytokines, such as IL-2, IL-3, GM-CSF, IFN-γ and TNF-α. Besides its anti-inflammatory capabilities, IL-10 also regulates the production of natural killer cells and lymphocytes. Due to its multiple roles, proper function of IL-10 is required to maintain a healthy immune system. Improper function of IL-10 can result in disease development, as over-expression of IL-10 has been linked to lymphoma, whereas IL-10 deficiency has implications for Crohn’s disease.
      References
      Product Information
      Components
      • IL-10 Coated Beads
      • Standard Diluent
      • IL-10 Detection Antibody
      • Assay Buffer
      • Human IL-10 Standard
      • SMC™ Human IL-10 Immunoassay Kit Instructions
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      Precision, %
      • Low-end CV% Range: 1-12%
      • Low-end CV% Average: 6%
      Applications
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-10 Immunoassay Kit unparalleled performance in the quantification of IL-10 in human EDTA plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-10
      Accuracy
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Erenna SMC™ Human IL-10 Immunoassay Kit Instructions

      SMC™ Human Interleukin 10 (IL-10)
      IMMUNOASSAY KIT SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      Singulex Erenna® Immunoassay System

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits