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AB947-K | Anti-Apolipoprotein E (1L)

AB947-K
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      Overview

      Replacement Information
      Description
      Catalogue NumberAB947-K
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Apolipoprotein E (1L)
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-Apolipoprotein E (1L)
      References
      Product Information
      FormatSerum
      Control
      • Liver tissue
      PresentationDelipidated goat antiSerum, 0.2 μm filtered. Liquid in Tris-saline, pH 7.4 with 0.1% sodium azide as preservative.
      Applications
      ApplicationDetect Apolipoprotein E using this Anti-Apolipoprotein E Antibody validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot (Schwab, 1996): 1:1,000-1:2,000

      Immunohistochemistry (Summers, 1998; Wisniewski & Frangione, 1992; Schwab, 1996)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human apolipoprotein E.
      HostGoat
      SpecificityMonospecific for Apolipoprotein E by immunoelectrophoresis when tested against pooled human plasma and twice concentrated pooled human serum. Reacts with Apo E isoforms E2, E3 and E4 by Western blot.
      Species Reactivity
      • Human
      • Primate
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryChylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
      Gene Symbol
      • APOE
      • Apo-E
      • apoprotein
      • MGC1571
      • AD2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02649 # Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.
      SIZE: 317 amino acids; 36154 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.
      PTM: Synthesized with the sialic acid attached by O-glycosidic linkage and is subsequently desialylated in plasma. & Glycated in plasma VLDL of normal subjects, and of hyperglycemic diabetic patients at a higher level (2-3 fold).
      DISEASE: SwissProt: P02649 # Defects in APOE are a cause of hyperlipoproteinemia type III [MIM:107741]; also known as familial dysbetalipoproteinemia. Individuals with hyperlipoproteinemia type III, are clinically characterized by xanthomas, yellowish lipid deposits in the palmar crease, or less specific on tendons and on elbows. The disorder rarely manifests before the third decade in men. In women, it is usually expressed only after the menopause. The vast majority of the patients are homozygous for APOE*2 alleles. More severe cases of hyperlipoproteinemia type III have also been observed in individuals heterozygous for rare APOE variants. The influence of APOE on lipid levels is often suggested to have major implications for the risk of coronary artery disease (CAD). Individuals carrying the common APOE*4 variant are at higher risk of CAD. & The APOE*4 allele is associated with late onset Alzheimer disease 2 (AD2) [MIM:104310]. The APOE*4 allele is genetically associated with the common late onset familial and sporadic forms of Alzheimer disease (AD). Risk for AD increased from 20% to 90% and mean age at onset decreased from 84 to 68 years with increasing number of APOE*4 alleles in 42 families with late onset AD. Thus APOE*4 gene dose is a major risk factor for late onset AD and, in these families, homozygosity for APOE*4 was virtually sufficient to cause AD by age 80. The mechanism by which APOE*4 participates in pathogenesis is not known. & Defects in APOE are a cause of sea-blue histiocyte disease [MIM:269600]; also called sea-blue histiocytosis. This disorder is characterized by splenomegaly, mild thrombocytopenia and, in the bone marrow, numerous histiocytes containing cytoplasmic granules which stain bright blue with the usual hematologic stains. The syndrome is the consequence of an inherited metabolic defect analogous to Gaucher disease and other sphingolipidoses.
      SIMILARITY: SwissProt: P02649 ## Belongs to the apolipoprotein A1/A4/E family.
      Molecular Weight34 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

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      Catalogue Number Description  
      AB947 Anti-Apolipoprotein E Antibody Show Pricing & Availability

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      Categories

      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Neurological Proteins
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Secondary Bulk Antibodies > Anti-Human Antibodies & Conjugates > Goat Anti-Human
      Life Science Research > Antibodies and Assays > Primary Antibodies
      IVD/OEM Materials & Reagents > Bulk & Custom Antibodies > Primary Bulk Antibodies by Target > Lipoproteins