MABN1792 Anti-Sortilin Antibody, clone F11

MABN1792
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M IH(P), ICC, WB, ELISA M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN1792
      DescriptionAnti-Sortilin Antibody, clone F11
      Alternate Names
      • Sortilin
      • 100 kDa NT receptor
      • Glycoprotein 95
      • Gp95
      • Neurotensin receptor 3
      • NT3
      • NTR3
      Background InformationSortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the family of sorting receptors that are characterized by the Vps10p domain, which creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. These family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reported to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit low levels of PCSK9 in plasma and its subcellular localization in hepatocytes is also altered. Sortilin is also found to co-localize with amyloid precursor protein (APP) in neurites and it facilitates APP processing toward production of sAPPα, possibly by optimizing APP as substrate for α-secretase cleavage. Sortilin is initially produced with a signal (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region(a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Like the other family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Sortilin Antibody, clone F11 is an antibody against Sortilin Antibody for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and ELISA.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      • ELISA
      Application NotesImmunocytochemistry Analysis: A representative lot detected cellular localization of endogenous sortilin in isolated hippocampal neurons from new born (P0) wild-type, but not Sort1−/− C57BL/6J mice (Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71).
      Immunocytochemistry Analysis: Representative lots detected cellular localization of exogenously expressed sortilin in transfected HEK293 cells by fluorescent immunocytochemistry (Larsen, J.V., et al. (2014). Biochem J. 457(2):277-288; Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71; Larsen, J.V., et al. (2010). Mol. Cell. Biol. 30(17):4175-4187; Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
      ELISA Analysis: A representative lot was employed as the detection antibody in sandwich ELISA. A positive correlation was found between PCSK9 and soluble sortilin in human serum samples from healthy individuals (Gustafsen, C., et al. (2014). Cell Metab. 19(2):310-318).
      Western Blotting Analysis: A representative lot detected different distribution of Sortilin than SorLA among subcellular fractions obtained by gradient centrifugation (Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
      Biological Information
      ImmunogenRecombinant protein corresponding to the extracellular domain of human Sortilin.
      EpitopeExtracellular domain
      CloneF11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SORT1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~91 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human cerebral cortex tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sortilin in human cerebral cortex tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Sortilin Antibody, clone F11 MSDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Sortilin Antibody, clone F11 Certificates of Analysis

      TitleLot Number
      Anti-Sortilin, clone F11 - 31920473192047
      Anti-Sortilin, clone F11 - 32802653280265
      Anti-Sortilin, clone F11 -Q2611290Q2611290
      Anti-Sortilin, clone F11 Monoclonal Antibody2979892
      Anti-Sortilin, clone F11 Monoclonal Antibody3085614

      References

      Reference overviewPub Med ID
      Human sorCS1 binds sortilin and hampers its cellular functions.
      Larsen, JV; Hermey, G; Sørensen, ES; Prabakaran, T; Christensen, EI; Gliemann, J; Madsen, P; Petersen, CM
      The Biochemical journal  457  277-88  2014

      Show Abstract
      24128306 24128306
      The hypercholesterolemia-risk gene SORT1 facilitates PCSK9 secretion.
      Gustafsen, C; Kjolby, M; Nyegaard, M; Mattheisen, M; Lundhede, J; Buttenschøn, H; Mors, O; Bentzon, JF; Madsen, P; Nykjaer, A; Glerup, S
      Cell metabolism  19  310-8  2014

      Show Abstract
      24506872 24506872
      Sortilin and SorLA display distinct roles in processing and trafficking of amyloid precursor protein.
      Gustafsen, C; Glerup, S; Pallesen, LT; Olsen, D; Andersen, OM; Nykjær, A; Madsen, P; Petersen, CM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  64-71  2013

      Show Abstract
      23283322 23283322
      Sortilin facilitates signaling of ciliary neurotrophic factor and related helical type 1 cytokines targeting the gp130/leukemia inhibitory factor receptor beta heterodimer.
      Larsen, JV; Hansen, M; Møller, B; Madsen, P; Scheller, J; Nielsen, M; Petersen, CM
      Molecular and cellular biology  30  4175-87  2010

      Show Abstract
      20584990 20584990
      Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA.
      Nielsen, MS; Gustafsen, C; Madsen, P; Nyengaard, JR; Hermey, G; Bakke, O; Mari, M; Schu, P; Pohlmann, R; Dennes, A; Petersen, CM
      Molecular and cellular biology  27  6842-51  2007

      Show Abstract
      17646382 17646382

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies