48-610MAG MILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

48-610MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641) H Mplex Luminex xMAP
      Description
      Catalogue Number48-610MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay
      OverviewThe STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Panel TypeCell Signaling
      Applications
      ApplicationThe analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
      Key Applications
      • Multiplexing
      Application Notes• An overnight (4°C) incubation is recommended for best results.
      • This assay requires 25 µL diluted cell lysate per well.
      • This kit must be run using Assay Buffer 1 (provided).
      • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • STAT1 (Tyr701)
      • STAT2 (Tyr690)
      • STAT3 (Tyr705)
      • STAT5A/B (Tyr694/699)
      • STAT6 (Tyr641)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: 5 Plex STAT Phosphoprotein Magnetic Bead Kit

      MILLIPLEXMAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay MSDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      Custom Immunoassay Menu of Services
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single

      Data Sheet

      Title
      Full Service Custom-built Assays

      Posters

      Title
      MILLIPLEX MAP: Exercise, Signaling, and Your Brain
      Biological Evaluation of Human PBMC and Th17 cells using MILLIPLEX® MAP Cell Signaling and Cytokine Panels
      MILLIPLEX® MAP Cell Signaling Wall Chart (MM)