03-0089-01 SMC® Human Interleukin 6 (IL-6)
IMMUNOASSAY KIT

03-0089-01
96 well assay  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-6 H Single Molecule Counting
      Description
      Catalogue Number03-0089-01
      Trade Name
      • SMC®
      DescriptionSMC® Human Interleukin 6 (IL-6)
      IMMUNOASSAY KIT
      OverviewThe SMC® IL-6 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
      Background InformationInterleukin-6 (IL-6) is a pro-inflammatory cytokine secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPs), which trigger the innate immune response and initiate inflammatory cytokine production. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also called a “myokine,” a cytokine produced from muscle that increases in response to muscle contraction. Additionally, osteoblasts secrete IL-6 to stimulate osteoclast formation.
      References
      Product Information
      Components
      • Assay Buffer
      • IL-6 Coated Beads
      • Human IL-6 Standard Diluent
      • IL-6 Detection Antibody
      • Human IL-6 Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      • SMC® Human IL-6 Immunoassay Kit Instructions
      Precision, %
      • Low-end CV% Range: 2 - 8%
      • Low-end CV% Average: 6%
      Applications
      ApplicationThe sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Human IL-6 Immunoassay Kit unparalleled performance in quantification of IL-6 in human EDTA plasma.
      Key Applications
      • Single Molecule Counting
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-6
      Accuracy
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Erenna SMC™ Human IL-6 Immunoassay Kit

      SMC® Human Interleukin 6 (IL-6)
      IMMUNOASSAY KIT MSDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      SMCxPRO™ Immunoassay System - Sensitivity You can Count On
      Singulex Erenna® Immunoassay System

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Application Note: Improved performance with conversion from ELISA to an Erenna® Immunoassay (MM)
      Quality Control Ranges: Erenna SMC™ Human IL-6 Controls
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits