A Stable RP Stationary Phase, Optimized for Applications up to pH12

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Aluspher® 100 RP-select B HPLC columnMerck’s Aluspher® RP-select B HPLC column is an alkali-stable reversed-phase stationary phase designed for the chromatographic separation of neutral, basic and acidic compounds under neutral conditions. Due to its stability in the pH range of 2-12, Aluspher® RP-select B allows the use of basic eluents, such as NaOH. This suppresses the ionization of basic compounds, and thus avoids peak tailing. Furthermore, advanced formulation technology has enabled the production of spherical alumina particles as a base for Aluspher® 100 RP-select B. The combination of alumina with alkaline eluents has led to new applications in HPLC.

Specifications of Aluspher®

Separation of Pharmaceuticals with
Non-Buffered Eluent

Column
Aluspher® 100 RP-select B (5 μm)
LiChroCART® 250-4
Mobile phase
Acetonitrile/Water 35/65 (v/v)
Flow rate
1.0 ml/min
Detection
UV 220 nm
Temperature Room temperature
Sample 1. Levamisole
2. 5-Methyl-5-phenyldantoin (MPH)
3. 5-Phenyl-5-(2-pyridyl)-hydantoin (PPH)
4. 5-(p-Methylphenyl)-5-phenylhydantoin (MPH)
5. Diazepam
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-Separation of pharmaceuticals with non-buffered eluent-250x260-10292013.jpg

Separation of Amphetamine Compounds

Column
Aluspher® 100 RP-select B (5 μm)
LiChroCART® 250-4
Mobile phase
Methanol/0.025 M NaOH 25/75 (v/v)
Flow rate
1.0 ml/min
Detection
UV 215 nm
Temperature Room temperature
Sample 1. Amphetamine
2. p-Methoxyamphetamine (PMA)
3. Methylendioxyamphetamine (MDA)
4. Methamphetamine
5. Methylendioxymethamphetamine (MDMA)
6. Ethylamphetamine
7. Methylendioxyethylamphetamine (MDEA)
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-Separation of amphetamine compounds-250x242-10292013.jpg

Separation of Beta-Blockers
with Alkaline Eluents

Column
Aluspher® 100 RP-select B (5 μm)
LiChroCART® 250-4
Mobile phase
Methanol/0.025 M NaOH 50/50 (v/v)
Flow rate
1.0 ml/min
Detection
UV 215 nm
Temperature Room temperature
Sample 1. Sotalol
2. Atenolol
3. Metoprolol
4. Bisoprolol
Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-Separation of beta-blockers with alkaline eluents-250x242-10292013.jpg
 
 
 
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