Enzymes for Culture Media


Enzymes for Efficient Inactivation of
Beta-Lactam Antibiotics

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β-Lactamases in Culture Media or as Enzyme Preparations

Merck provides solid culture media which are supplemented with β-lactamases (see also ICR Settle Plates and ICR Contact Plates). These enzymes efficiently inactivate a broad spectrum of β-lactam antibiotics, including penicillins, cephalosporins and carbapenems. Alternatively, lyophilized or liquid, highly potent preparations of these β-lactamase enzymes are also available. These Merck products are suitable for sterility and bioburden testing as well as for environmental monitoring.

β-Lactamases Are Able to Inactivate

  • Penicillins (e.g. Ampicillin, Penicillin and Mezlocillin)
  • Cephalosporins from the 1st to the 5th generation
  • Carbapenems (e.g. Ertapenem, Imipenem and Meropenem)

The β-lactamase products provide high enzymatic activity, with culture media containing 1.000 IU/I cephalosporinase (cephase) and 10.000 UI/I penicillinase (penase).

The Need for Antibiotics Inactivation

Wherever culture media are employed in the manufacturing of antibiotics they need to contain inactivating substances to neutralize efficiently these antibiotics to ensure that any sensitive contaminant microorganisms maintain their ability to grow.

In manufacturing facilities where antibiotics are produced, β-lactamase enzymes such as penicillinase (penase) and cephalospopinase (Cephase) are contained in the monitoring media to inactivate β-Lactam antibiotics. Produced antibiotics must also be inactivated prior to sterility testing to allow for the detection of potential contaminants which are sensitive to the antibiotic.

Enzymes for Culture Media

Enzymes for Culture Media

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Environmental Monitoring Brochure

Environmental Monitoring

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