119109 Luciferin-Luciferase

119109
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.





      Firefly luciferin-luciferase reagent for the ATP assay. Essential component of the ATP Assay Kit (Cat. No. 119107). Luciferase catalyzes the oxidation of D-luciferin to oxyluciferin in the presence of ATP and oxygen. As the reaction proceeds, light is produced.
      Catalogue Number119109
      Brand Family Calbiochem®
      References
      ReferencesDeLuca, M., et al. 1979. Anal. Biochem. 95, 194.
      Chappelle, E., et al. 1978. Methods Enzymol. 57, 65.
      Product Information
      Activity≥3,200,000 RLU's per 10 µl reconstituted solution at 2 x 10⁻⁸ M ATP using a Monolight 3010 Luminometer
      FormLyophilized
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at -70°C or for up to 8 h at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      119109

      References

      Reference overview
      DeLuca, M., et al. 1979. Anal. Biochem. 95, 194.
      Chappelle, E., et al. 1978. Methods Enzymol. 57, 65.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-April-2008 RFH
      DescriptionFirefly luciferin-luciferase reagent for the ATP assay. Essential component of the ATP Assay Kit (Cat. No. 119107). Luciferase catalyzes the oxidation of D-luciferin to oxyluciferin in the presence of ATP and oxygen. As the reaction proceeds, light is produced.
      FormLyophilized
      Activity≥3,200,000 RLU's per 10 µl reconstituted solution at 2 x 10⁻⁸ M ATP using a Monolight 3010 Luminometer
      SolubilityReconstitute in 500 µl 25 mM HEPES buffer, 10 mM MgCl₂, 0.02% NaN₃, pH 7.5.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at -70°C or for up to 8 h at 4°C.
      Toxicity Standard Handling
      ReferencesDeLuca, M., et al. 1979. Anal. Biochem. 95, 194.
      Chappelle, E., et al. 1978. Methods Enzymol. 57, 65.