Chromolith® CapRod®
Characterization of Chromolith® CapRod®
- The cross section shows the bimodal pore structure of Chromolith® CapRod® with macropores at ~ 2 µm (1 µm for “HighResolution” products) and mesopores at 13 nm.
The new Chromolith® CapRod® capillary column is designed for the efficient and selective separation of peptides and protein digests and is especially suited for capillary or nano-LC. Based on proprietary sol-gel technology, highly porous monolithic rods of pure silica with a unique pore structure are formed. Each column has a macropore and a mesopore structure which gives it a very high porosity. The macropores form a network of pores through which the eluent can rapidly flow, hence dramatically reducing separation time. The mesopores form the fine-pore structure of the capillary interior and create a very large surface area onto which adsorption of the target molecule can occur. The Chromolith® CapRod® analytical capillary columns are supplied complete with sleeves and standard 1/16” PEEK fittings to allow for direct coupling to a UV detector or mass spectrometer.
Specifications of Chromolith® CapRod®
| Sorbent characteristics | Monolithic silica gel |
| Column inner diameter | 0.05 (50 μm), 0.1 mm (100 μm) and 0.2 mm (200 μm) |
| Column length | 150 mm, 300 mm |
| Surface modification | RP-8 endcapped, RP-18 endcapped |
| Macropore size | 2 μm (1 μm for “HighResolution” products) |
| Mesopore size | 13 nm |
| Surface area | 300 m2/g |
Recommended use and flow rate ranges
| Recommended use | RP-18e 150 x 0.05 |
RP-8e 150 x 0.1 |
RP-18e 50 x 0.1 Trap |
RP-18e 150 x 0.1 |
RP-18e 300 x 0.1 |
RP-18e 150 x 0.1 HR |
RP-18e 50 x 0.2 Trap |
RP-18e 150 x 0.2 |
RP-18e 150 x 0.2 HR |
| Separation of small molecules | • | • | • | • | • | • | • | ||
| Separation of peptides | • | • | • | • | • | • | • | • | |
| Separation of proteins | • | ||||||||
| Micro ESI | • | • | • | • | • | • | • | ||
| Nano ESI | • | • | • | • | • | • | |||
| High Resolution | • | • | |||||||
| Flow rates [μL/min] | 0.2 - 0.8 | 0.4 - 3 | 1 - 10 | 0.4 - 3 | 0.2 - 1.5 | 0.1 - 0.4 | 10 – 50 | 5 – 20 | 0.5 - 2 |
| Max. back-pressure [bar] | 200 | 200 | 200 | 200 | 200 | 218 | 218 | 218 | 218 |
Separation repeatability for biological compounds
- Excellent repeatability / reproducibility on monolithic silica capillaries
- Long lifetime of capillary due to high mechanical stability of porous silica network
| Column | Chromolith® CapRod®, RP-18e 150 mm x 0.1 mm |  |
|
| Mobile phase |
A: 2% ACN, 0.1% FA B: 80% ACN, 0.08% FA |
||
| Gradient | 2% to 40% B in 35 min | ||
| Flow rate | 3 μL/min | ||
| Sample | Cytochrom C digest | ||
Ordering Information – Chromolith® CapRod®
| 50 x 0.1 mm | 50 x 0.2 mm | 150 x 0.05 mm | 150 x 0.1 mm | 150 x 0.2 mm | 300 x 0.1 mm | |
| Chromolith® CapRod® RP-8e |
1.50400.0001 | |||||
| Chromolith® CapRod® RP-8e Trap |
1.52031.0001 | |||||
| Chromolith® CapRod® RP-18e |
1.50403.0001 | 1.50402.0001 | 1.50405.0001 | 1.50424.0001 | ||
| Chromolith® CapRod® RP-18e HighResolution |
1.50404.0001 | 1.50407.0001 | ||||
| Chromolith® CapRod® RP-18e Trap |
1.50426.0001 | 1.50409.0001 |
Further suggested reading
