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Chromolith HPLC

Chromolithy® RP-8 endcapped HPLC column application

Chromolith® RP-8 endcapped HPLC column

Benefits in working with Chromolith® RP-8 endcapped HPLC columns

  • High throughput at high flow rates with the best overall column quality
  • The possibility to use flow gradients
  • Added column performance by column coupling
  • A rigid structure for a longer lifetime
  • Lower matrix sensitivity

 

Ordering Information – Chromolith® RP-8 endcapped HPLC column

  100x4.6 mm
Chromolith® Performance RP-8 endcapped 1.51468.0001

 

 
Separation examples on Chromolith® Performance RP-8 endcapped 100-4.6 mm
Column Chromolith® Performance RP-8 endcapped, 100-4.6 mm Separation on Chromolith® Performance RP-8 endcapped
Mobile phase A: Acetonitrile/ water 90/ 10 + 0.1% TFA
B: 0.1% TFA in water
Gradient Time/min
0.0
1.0
3.0
%A
45
90
90
%B
55
10
10
 
Flowrate 2 mL/min
Pressure 30 - 40 bar
Detection 214 nm
Temperature ambient
Injection volume 30 μL
Sample 1. (Sar1, Ala8)-Angiotensin II
2. (Sar1, Ile8)-Angiotensin II
3. Angiotensin                       
87 μg/mL
87 μg/mL
47 μg/mL
Separation examples on Chromolith® Performance RP-8 endcapped 100-4.6 mm
Column Chromolith® Performance RP-8 endcapped, 100-4.6 mm Separation on Chromolith® Performance RP-8 endcapped
Mobile phase A: Acetonitrile
B: 20mM NaH2PO4 pH 2.5
Gradient   Time/min
0
0.5
8.5
9.1
16
% A
2
18
18
32
32
   
Fow rate 1 mL/min
Pressure 23 bar
Detection 220 nm
Temperature ambient
Injection volume 5 μL
Sample 1. Malic acid
2. Succinic acid
3. Glutaric acid
4. 3,4-Dihydroxy-cinnamic acid
5. 4-Hydroxy-cinnamic acid
6. Sorbic acid
7. Benzoic acid
8. 2-Hydroxybenzoic acid
9. Cinnamic acid                           
0.92 mg/mL
1.70 mg/mL
1.20 mg/mL
0.02 mg/mL
0.03 mg/mL
0.20 mg/mL
0.04 mg/mL
0.15 mg/mL
0.04 mg/mL

 

Ordering Information – Chromolith® RP-8 endcapped HPLC column

  100x4.6 mm
Chromolith® Performance RP-8 endcapped 1.51468.0001

 

Further suggested reading 

Chromolith® HPLC  
  Detailed information about Chromolith®
HPLC columns
 
  Chromolith® CapRod®  
  Chromolith® column coupler  
  Chromolith® HPLC FastGradient  
  Chromolith® HPLC guard cartridges  
  Chromolith® NH2  
  Chromolith® Prep  
  Chromolith® RP-8 endcapped  
  Example applications using
Chromolith® RP-8 endcapped
HPLC columns
  Chromolith® RP-18 endcapped  
  Chromolith® RP-18 endcapped HighResolution  
  Chromolith® Si  
  Chromolith® SemiPrep  

© Merck KGaA, Darmstadt, Germany, 2014


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