Chromolithy® RP-8 endcapped HPLC column application
Benefits in working with Chromolith® RP-8 endcapped HPLC columns
- High throughput at high flow rates with the best overall column quality
- The possibility to use flow gradients
- Added column performance by column coupling
- A rigid structure for a longer lifetime
- Lower matrix sensitivity
Ordering Information – Chromolith® RP-8 endcapped HPLC column
| 100x4.6 mm | |
| Chromolith® Performance RP-8 endcapped | 1.51468.0001 |
| Separation examples on Chromolith® Performance RP-8 endcapped 100-4.6 mm | ||||||
|---|---|---|---|---|---|---|
| Column | Chromolith® Performance RP-8 endcapped, 100-4.6 mm |  |
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| Mobile phase | A: Acetonitrile/ water 90/ 10 + 0.1% TFA B: 0.1% TFA in water |
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| Gradient | Time/min 0.0 1.0 3.0 |
%A 45 90 90 |
%B 55 10 10 |
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| Flowrate | 2 mL/min | |||||
| Pressure | 30 - 40 bar | |||||
| Detection | 214 nm | |||||
| Temperature | ambient | |||||
| Injection volume | 30 μL | |||||
| Sample | 1. (Sar1, Ala8)-Angiotensin II 2. (Sar1, Ile8)-Angiotensin II 3. Angiotensin |
87 μg/mL 87 μg/mL 47 μg/mL |
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| Separation examples on Chromolith® Performance RP-8 endcapped 100-4.6 mm | |||||
|---|---|---|---|---|---|
| Column | Chromolith® Performance RP-8 endcapped, 100-4.6 mm |  |
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| Mobile phase | A: Acetonitrile B: 20mM NaH2PO4 pH 2.5 |
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| Gradient | Time/min 0 0.5 8.5 9.1 16 |
% A 2 18 18 32 32 |
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| Fow rate | 1 mL/min | ||||
| Pressure | 23 bar | ||||
| Detection | 220 nm | ||||
| Temperature | ambient | ||||
| Injection volume | 5 μL | ||||
| Sample | 1. Malic acid 2. Succinic acid 3. Glutaric acid 4. 3,4-Dihydroxy-cinnamic acid 5. 4-Hydroxy-cinnamic acid 6. Sorbic acid 7. Benzoic acid 8. 2-Hydroxybenzoic acid 9. Cinnamic acid |
0.92 mg/mL 1.70 mg/mL 1.20 mg/mL 0.02 mg/mL 0.03 mg/mL 0.20 mg/mL 0.04 mg/mL 0.15 mg/mL 0.04 mg/mL |
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Ordering Information – Chromolith® RP-8 endcapped HPLC column
| 100x4.6 mm | |
| Chromolith® Performance RP-8 endcapped | 1.51468.0001 |
Further suggested reading
