Chromolith® SemiPrep - Perfect scale-up from analytical to preparative LC
Optimum separation at flow rates exceeding 40 mL/min
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with very high separation performance.
They are the ideal alternative to particulate columns with a 10 mm i.d. (and even 21.2 mm). The selectivity of Chromolith® RP-18 endcapped SemiPrep columns is comparable to common RP-18 endcapped reversed phase columns. It is an excellent tool to solve separation problems regarding non-polar basic and acidic compounds as well as peptides.
The Chromolith® SemiPrep columns have the same bimodal porous silica rod structure as the Chromolith® analytical columns with a 4.6 mm inner diameter. The macropores are 2 μm in diameter and the mesopores are 13 nm.
| Chromolith® SemiPrep benefits |
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Specifications of Chromolith® SemiPrep 100-10 mm
| Silica type | High-purity (99.999%) |
| Particle size | Monolithic |
| Macropore size | 2 μm |
| Mesopore size | 13 nm (130 Å) |
| Pore volume | 1.0 mL/g |
| Total porosity | > 80% |
| Surface area | 300 m2/g |
| Surface modification | RP-18 endcapped |
| Selectivity equivalent to | L1 (USP) |
| Carbon content | 18% |
| Surface coverage | 3.6 μmol/m2 |
| Mobile phase compatibility | all standard HPLC solvents may be used with the following restrictions |
| Max. dichloromethane conc. | 5% |
| Max. tetrahydrofuran conc. | 50% |
| Max. dimethylsulphoxide DMSO | 5% but OK as sample solvent |
| pH range | 2-7.5 |
| Max pressure | 150 bar for 10 mm columns |
| Max temperature | 45°C |
Optimum separation at various flow rates
Chromolith® SemiPrep RP-18e 100-10 mm i.d. columns combine high separation speed with very high separation performance. They are the ideal alternative to particulate columns with 10 mm i.d. (and even 21.2 mm).
Separation of a standard mixture
| Acetonitrile/water 60/40, data for anthracene (3rd peak) | |||
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| Mobile phase | Acetonitrile/ water 60/40 |
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| Flow rate | 2 mL/min | ||
| Detection | UV 254 nm | ||
| Temperature | ambient | ||
| Injection volume | 5 μL | ||
| Sample | 1. Thiourea 2. Progesterone 3. Anthracene |
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Ordering information – Chromolith® SemiPrep 100-10 mm
| 100x10 mm | |
| Chromolith® SemiPrep RP-18 endcapped | 1.52016.0001 |
Further suggested reading
