Chromolith® SemiPrep - Perfect scale-up from analytical to preparative LC
Optimum separation at flow rates exceeding 40 mL/min
Chromolith® SemiPrep 10 mm i.d. columns combine high separation speed with very high separation performance.
They are the ideal alternative to particulate columns with a 10 mm i.d. (and even 21.2 mm). The selectivity of Chromolith® RP-18 endcapped SemiPrep columns is comparable to common RP-18 endcapped reversed phase columns. It is an excellent tool to solve separation problems regarding non-polar basic and acidic compounds as well as peptides.
The Chromolith® SemiPrep columns have the same bimodal porous silica rod structure as the Chromolith® analytical columns with a 4.6 mm inner diameter. The macropores are 2 μm in diameter and the mesopores are 13 nm.
|Chromolith® SemiPrep benefits|
Specifications of Chromolith® SemiPrep 100-10 mm
|Silica type||High-purity (99.999%)|
|Macropore size||2 μm|
|Mesopore size||13 nm (130 Å)|
|Pore volume||1.0 mL/g|
|Total porosity||> 80%|
|Surface area||300 m2/g|
|Surface modification||RP-18 endcapped|
|Selectivity equivalent to||L1 (USP)|
|Surface coverage||3.6 μmol/m2|
|Mobile phase compatibility||all standard HPLC solvents may be used with the following restrictions|
|Max. dichloromethane conc.||5%|
|Max. tetrahydrofuran conc.||50%|
|Max. dimethylsulphoxide DMSO||5% but OK as sample solvent|
|Max pressure||150 bar for 10 mm columns|
Optimum separation at various flow rates
Chromolith® SemiPrep RP-18e 100-10 mm i.d. columns combine high separation speed with very high separation performance. They are the ideal alternative to particulate columns with 10 mm i.d. (and even 21.2 mm).
Separation of a standard mixture
|Acetonitrile/water 60/40, data for anthracene (3rd peak)|
|Flow rate||2 mL/min|
|Detection||UV 254 nm|
|Injection volume||5 μL|
Ordering information – Chromolith® SemiPrep 100-10 mm
|Chromolith® SemiPrep RP-18 endcapped||1.52016.0001|
Further suggested reading