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Excellent separations with ZIC®-HILIC HPLC columns

SeQuant® ZIC®-HILIC HPLC columns

SeQuant® HILIC HPLC columns have documented excellent selectivity for straightforward separation of polar hydrophilic compounds in HPLC and LC-MS methods. These columns carry densely bonded, truly zwitterionic functional groups with a charge balance of 1:1. Separation is achieved by hydrophilic partitioning combined with weak ionic interactions for maximum selectivity, high loadability and easy optimization of methods.

 

The comprehensive product range of SeQuant® ZIC®-HILIC columns covers dimensions from capillary through analytical to semi-preparative. Its favorable retention and robust performance have made SeQuant® ZIC®-HILIC one of the most popular HILIC columns all over the world.

“In particular, the zwitterionic phase ZIC-HILIC showed better selectivity for the test samples than the other phases.”
Kawachi et al., J. Chromatogr. A 1218 (2011) 5903-5919

 

This citation is taken from an article published by distinguished Japanese scientists. They performed comprehensive screening to compare the performance of 15 different HILIC columns. Their conclusion was that the selectivity of SeQuant® ZIC®-HILIC was higher and more versatile than that of all other stationary phases.

Alternative choices for separation of polar hydrophilic compounds include SeQuant®  ZIC®-cHILIC with complementary selectivity and SeQuant® ZIC®-pHILIC for higher pH stability. Find out more on the corresponding webpages giving an overview of SeQuant® HILIC columns.

 

Read on: What is HILIC?Product RangeApplicationsTechnical Support

What is HILIC?

With HILIC you combine the benefits of aqueous mobile phases like in reversed-phase HPLC with separation based upon normal phase principles where more polar compounds respond with higher retention resulting in more efficient separation. More details are available on our webpages giving an overview of SeQuant® HILIC columns.

Visit the HILIC Technology Tutorial or request your free copy of ‘A Practical Guide to HILIC’ to learn more about the options offered by the HILIC technique and its advantages in separating polar hydrophilic substances.

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Product Range

SeQuant® ColumnCharge arrangement and functional groupFeaturesBase particleParticle sizePore sizeColumn types
SeQuant®
ZIC®-HILIC
SeQuant® ZIC®-HILIC
Sulfobetaine
Well-known for high-performance selectivity and robustness silica 3.5 µm,
5 µm
100 Å, 200 Å

analytical,
capillary,
semi-prep,
guards

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Applications

The use of SeQuant® ZIC®-HILIC has been recommended by US FDA (United States Food and Drug Administration) in its methods for the analysis of melamine and related potential contaminations.

The ZIC®-HILIC column has been used in numerous methods for a variety of compounds such as acids and bases, anions and cations, carbohydrates, metabolites, metal complexes, amino acids, peptides, protein digests and many more.

 

Separation of cyanuric acid melamine food contaminants
Column ZIC®-HILIC 150 x 2.1 mm, 5 μm,
200 Å (1.50454.0001)
SeQuant® ZIC®-HILIC
Mobile phase A: 95% acetonitrile 
in 0.1% aqueous formic acid
B: 50% acetonitrile 
in 20 mM aqueous ammonium             
formate
Gradient Time

0
4.2
8.0
8.5
9.0
11.0
11.2
13.0
14.0
A

100
100
65
65
25
25
100
100
100
B

0
0
35
35
75
75
0
0
0

Flow rate
[mL/min]
0.4
0.4
0.4
0.4
0.4
0.4
0.6
0.6
0.4

Detection MS/MS, -ESI and +ESI
Temperature 30°C
Inj. volume 5 μL
Sample Standard in mobile phase
(7 ng/mL) equivalent to 1 μg/g
Courtesy of David N. Heller, FDA Center for Veterinary Medicine, Laurel, MD 20708 USA Reference David N. Heller and Cristina B. Nochetto, Rapid Commun. Mass Spectrom., 22 (2008)
3624-3632. DOI: 10.1002/rcm.3779
Separation of amino acids
Column ZIC®-HILIC 150 x 4.6 mm, 3.5 μm, 100 Å
(1.50444.0001)
SeQuant® ZIC®-HILIC
Mobile phase 80% acetonitrile
20% ammonium acetate in water, 50 mM pH adjusted to 4.5 with formic acid
Flow rate 0.75 mL/min
Detection Refractive index, cell 9 μL, 40°C
Temperature 40°C       
Inj. volume 50 μL
Gradient 1. 2-Amino-5-Hydroxy benzoic acid
2. Tryptophan
3. Isoleucine
4. Methionine
5. Lycine
6. Threonine

100 ppm
100 ppm
100 ppm
100 ppm
100 ppm
100 ppm

Courtesy of Gora Sharangi, Merck India Application Lab
Bioanalysis of methyl malonic acid
Column ZIC®-HILIC 100 x 2.1 mm, 3.5 μm,100 Å
(1.50444.0001)
SeQuant® ZIC®-HILIC
Mobile phase A: 80% (v/v) acetonitrile
B: 20% (v/v) 100 mM NH4Ac buffer pH 4.5
Flow rate MS-ESI- with SIM at 117.2 m/z           
Drying-gas flow rate
Drying-gas temperature
Capillary voltage

10 L/min
300°C
3.0 kV
Detection Refractive index, cell 9 μL, 40°C
Inj. volume 4 μL      
Sample Precipitation-treated plasma sample containing 0.137 mol/L MMA
Reference    H-A Lakso, P. Appelblad, J. Schneede
Clin. Chem., 54 (2008) 2028
DOI: 10.1373/clinchem.2007.101253

 Some exemplary application notes using ZIC®-HILIC:

More examples of how you can solve analysis problems with ZIC®-HILIC HPLC columns can be found in the ‘Chromatography Application World’ or in the comprehensive collection of SeQuant® application notes.

 

SeQuant® ZIC®-HILIC SeQuant® ZIC®-HILIC  
Glycomics Peptide mapping

Technical Support

Use the free online tool to predict retention on SeQuant® ZIC®-HILIC columns.

For technical support on ZIC®-HILIC products or questions regarding application development, please consult the SeQuant® FAQ list or contact the SeQuant® Support Team.

Detailed instructions for using ZIC®-HILIC columns can be found in the documents listed below. More supportive documents can be found in the SeQuant® download area.

Every HPLC separation column by Merck Millipore is individually tested and delivered with a certificate documenting its performance.

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SeQuant® HPLC columns 
  Applications and technical support
  ZIC®-cHILIC
  ZIC®-HILIC
  ZIC®-pHILIC
  ZIC®-HILIC Retention Prediction

 

© Merck KGaA, Darmstadt, Germany, 2014


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