c-Kit ELISA Kit
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Merck Chemicals Ltd.
Padge Road, Beeston
NG9 2JR Nottingham
Phone: +44 (0)115 9430840
Fax: +44 (0)115 9430951
10 March 2014
|Species reactivity||human, not mouse, not rat|
|Sensitivity||< 15 pg/ml|
|Assay range||19.5 - 1250 pg/ml|
|Assay time||4 h|
|Sample type||Cell lysate|
|Kit contains||c-Kit Standard, Standard Diluent Buffer, c-Kit Antibody-Coated 96-Well Plate, Rabbit Anti-c-Kit Detector Antibody, Anti-Rabbit IgG-HRP Concentrate, HRP Diluent, Wash Buffer Concentrate, TMB, Stop Solution, Plate Covers and a user protocol.|
|Store and ship information|
|Storage||+2°C to +8°C|
Blue Ice Only
Multiple Toxicity Values, refer to MSDS
|Canadian export regulations||Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.|
The sensitivity of this ELISA was compared to immunoblotting using known quantities of c-Kit. The data show that the sensitivity of the ELISA is approximately 4X greater than that of immunoblotting. The bands shown in the immunoblot data were developed using a rabbit anti-c-Kit antibody and chemiluminescent detection.
Mo7e cells were treated with Stem Cell Factor (SCF) at varying concentrations (1 to 100 ng/ml) for 10 min, lysed, and quantitated in parallel for total c-Kit and c-Kit (pTyr823) expression. The amount of total c-Kit remains relatively constant, while the level of phosphorylation at Tyr823 increases with the dose of SCF. The results correlate very well with immunoblot of the same samples (inset).