Protein phosphorylation has emerged as a key event in the control of signal transduction as well as an important tool for studying disease pathways. Over the past 15 years, phosphorylation research has benefited from the increasingly sophisticated tools for inhibiting, activating, and detecting phosphorylation events.
PhosphoDetect™phospho-specific antibodies discriminate between phosphorylated and non-phosphorylated forms of proteins or amino acids with exquisite specificity. These phospho-specific antibodies are suitable for many applications, including Western blot analysis, immunofluorescence microscopy, ELISA, immunocytochemical assays, and flow cytometry, thus enabling researchers to study the dynamics of protein phosphorylation in vivo, in situ, and in real time. The PhosphoDetect brand is your guarantee that our phospho-specific antibodies meet the rigid quality standards you rely on for consistent experimental results.
Why PhosphoDetect™ Phospho-specific Antibodies from Calbiochem
- Higher specificity
Antibodies discriminate between phosphorylated and non-phosphorylated forms of proteins or amino acids with exquisite specificity.
- Suitable for a broad range of applications
Western blot analysis, immunofluorescence microscopy, ELISA, immunocytochemical assays, and flow cytometry.
- Consistent quality guarantee
PhosphoDetect brand is your guarantee that our phospho-specific antibodies meet the rigid quality standards you rely on for consistent experimental results!
|Over 200 phospho-specific antibodies directed to targets in the areas of neurobiology, apoptosis, cell cycle regulation, transcription factors, receptor tyrosine kinases, Ser/Thr Kinases, and more.
We also offer:
|A wide variety of phospho-specific ELISA assays targeted to many proteins of interest.
PhosphoDetect ELISA Kits
Detection of human Smad2, phosphorylated on Ser465/467, by immunoblotting. Sample: Whole cell lysatesfrom HepG2 cells treated with TGF- for 0-60 min. Primary antibodies: PhosphoDetect™ Anti-Smad2 (pSer465/467) Rabbit pAb (Cat. No. 566415) (1:1000) (upper panel) or total Anti-Smad2 (lpanel). Detection: chemiluminescence.
Detection of human VEGF Receptor 2/3 by staining paraffin sections. Sample: human colon carcinoma. Primary antibody: PhosphoDetect™ Anti-VEGF Receptor 2/3 (pAb-1) Rabbit pAb (Cat. No. PC460) (1/2000). Detection: DAB with methyl green counterstain.
Detection of human ATM phosphorylated at (Ser1981) by immunofluorescence. Samples: Untreated HeLa cells (left panel) and camptothecin-treated (10 M, 2 h) HeLa cells, fixed with 100% methanol (right panel). Primary antibody: PhosphoDetect™ Anti-ATM (pSer1981) Mouse mAb (10H11.E12) (Cat. No. DR1002) (1.5 g/ml). Detection: fluorescence.