Unambiguous results are easy to see with Merck Millipore’s new silver staining products
Two new products join the silver staining product range. The Silver plating kit acc. to von Kossa is designed for simple, reproducible detection of calcium deposits in tissue samples. For verification of the staining process, Merck Millipore introduces the ISOSLIDE® control slides series, control tissue slides that can be compared with a lab’s own specimens.
Silver plating kit for detection of calcium deposits
Detection of calcification in tissues can lead to successful diagnosis of metabolic problems or tumors (e.g. mamma carcinoma).
Tissue calcification has been implicated in problems with metabolic processes. It’s also present in tumors. Correct detection of calcium deposits contributes to accurate diagnosis. Silver plating to von Kossa is an excellent method for visualizing calcium deposits in tissue samples. In the von Kossa method, silver nitrate solution reacts with calcium compounds within the tissue sample. Calcium ions are replaced by silver ions. After exposure to light, calcium structures become clearly visible as brown-black structures. Nuclear fast-red is recommended as a counter stain.
While it offers incredible sensitivity, many things can interfere with the silver plating process. Fortunately, the Merck Millipore silver plating kit acc. to von Kossa solves the interference problem and is accompanied by a robust silver plating protocol. The kit makes detection of calcium both simple and reproducible.
The critical event in this silver plating process is the light exposure. That’s why detailed instructions, including a trouble-shooting guide, are included with the kit to insure your success.
|Article information||Cat. No.|
|Silver staining kit acc. to von Kossa||1.00362.0001|
|Contains reagents for staining up to 300 tissue specimens:
|Nuclear fast red-aluminum sulfate solution 0.1%||1.00121.0500|
Tissue sections for verification of reticular fiber staining
Sometimes uncertainty can arise when analyzing stained tissue samples. Having a control to compare against can help to increase confidence in your results and document the quality of your processes.
The ISOSLIDE® Reticulin control slides are intended for the silver plating of reticular fibers. The control slides can be stained easily and reliably using the Reticulin silver plating kit acc. to Gordon & Sweets. You can either use Merck Millipore’s Reticulin silver plating kit acc. to Gordon & Sweet or alternatively, your laboratory’s own method. By including a control slide during your staining process, you have a positive sample to compare with your own tissue specimens.
Each box contains 25 serial tissue slides prepared from the same block of animal tissue. The slides have a 5-year shelf life. One control slide is pre-stained using the Reticulin silver plating reaction, the other 24 slides are unstained. ISOSLIDE® control slides are also helpful when it comes to lab accreditation or audits. They are CE-certified, registered as IVD and subject to comprehensive quality and process documentation.
|Article information||Cat. No.|
|ISOSLIDE® Reticulin control preparations||1.00361.0001|
|Reticulin silver plating kit according to Gordon & Sweets||1.00251.0001|