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Chromolith HPLC

Chromolith® HPLC column coupler

Chromolith® HPLC column coupler

Chromolith® Performance RP-18 endcapped columns provide rapid high-quality separation of complex mixtures. But, if necessary, the separation efficiency can be increased by coupling several columns together, using the Chromolith® HPLC column coupler. With the column coupler it is possible to increase the plate count by coupling several columns in series. In this way, columns with a theoretical plate count that is significantly higher compared to any particulate column available are produced. At the same time the producing pressure is kept still well below the HPLC system limit.

This added column performance is the key to solve even very critical separation problems where resolution is the limiting factor. Therefore they are perfect for use to separate hitherto non-separable complex mixtures.

The table below shows a comparison between Chromolith® HPLC columns and particulate columns. As you can clearly see, the combination of two Chromolith® Performance RP-18 endcapped columns yields a column with a separation efficiency of 19,000 theoretical plates per column, which is usually the maximum for particulate columns.

 

Click here to review example applications with Chromolith® Si HPLC columns.

 

Typical column efficiency using the Chromolith® HPLC column coupler

Column Length [mm] Pressure* [bar]  Plate number per column [Anthracene] 
Chromolith® Performance 1x 100 30 10,000
Chromolith® Performance 2x 200 60 19,000
Chromolith® Performance 3x 300 90 27,000
Chromolith® Performance 4x 400 120 35,000
Chromolith® Performance 5x 500 150 41,000
Particulate column (5 μm) 250 220 18,500
Particulate column (3.5 μm) 150 400 19,000
* = 3 mL/min 75% acetonitrile, 25% water

Ordering Information – Chromolith® HPLC column coupler

Chromolith® column coupler 1.51467.0001

 

Further suggested reading 

Chromolith® HPLC  
  Detailed information about Chromolith®
HPLC columns
 
  Chromolith® CapRod®  
  Chromolith® column coupler  
    Example applications using
Chromolith® HPLC column coupler
  Chromolith® HPLC FastGradient  
  Chromolith® HPLC guard cartridges  
  Chromolith® NH2  
  Chromolith® Prep  
  Chromolith® RP-8 endcapped  
  Chromolith® RP-18 endcapped  
  Chromolith® RP-18 endcapped HighResolution  
  Chromolith® Si  
  Chromolith® SemiPrep  

© Merck KGaA, Darmstadt, Alemania, 2014


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